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This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition. Various methods to investigatedirectional cell growth and movements are presented in Chapters 1-20. Thesechapters contains experimental procedures to visualize and measure migrationbehaviors of different kinds of organisms, including chemotropism in thebudding yeast; cell growth and migration of D. discoideum; border cellmigration in Drosophila; chemotaxis of mouse and human neutrophils; andHIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,Second Edition also contains microscopy procedures for studying breast cancercell migration, tumor cell invasion in vivo, and axon guidance. The book concludeswith Chapters 21-27 describing methods that measure spatiotemporal dynamics ofsignaling components involved in chemotaxis; introduce imaging techniques, suchas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models ofexperimentally generated chemoattractant gradients. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Cutting edge and thorough,Chemotaxis: Methods and Protocols, Second Edition is a valuable resource foranyone who is interested in the diverse methodologies that are propellingchemotaxis research forward. 

Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.