Bioluminescence

Methods and Protocols

AvSung Bae Kim

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 199 kr

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Beskrivning

This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.

Produktinformation

Utgivningsdatum:2018-05-31
Mått:178 x 254 x 25 mm
Vikt:656 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:314
Upplaga:3
Förlag:Humana Press Inc.
ISBN:9781493981465

Utforska kategorier

Innehållsförteckning

Label-free Cell Phenotypic Identificationof D-Luciferin as an Agonist for GPR35.- Synthetic BioluminescentCoelenterazine Derivatives.- Molecular Cloning of SecretedLuciferases from Marine Planktonic Copepods.- How to Fabricate FunctionalArtificial Luciferases for Bioassays.- QuantumYield Determination Based on Photon Number Measurement, Protocols for FireflyBioluminescence Reactions.- Bioluminescent Ligand-ReceptorBinding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of AminopeptidaseN in vitro and in vivo.- Firefly Luciferase-basedSequential Bioluminescence Resonance Energy Transfer (BRET)-FluorescenceResonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Changewith a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast andMammalian Cells.- A Protein–Protein InteractionAssay FlimPIA Based on the Functional Complementation of Mutant FireflyLuciferases.- Single-chain Probes for IlluminatingAndrogenicity of Chemicals.- MulticolorImaging of Bifacial Activities of Estrogens.- CircularPermutation Probes for Illuminating Phosphorylation of Estrogen Receptor.- Fabricationof Molecular Strain Probes for Illuminating Protein–Protein Interactions.- AnALuc-based Molecular Tension Probe for Sensing Intramolecular Protein–Protein Interactions.- Live Cell Bioluminescence Imagingin Temporal Reaction of G Protein-coupled Receptor for High-throughput Screeningand Analysis.- Imaging Histone Methylations inLiving Animals.- Preparation and Assay of Simple Light off Biosensor Based on ImmobilizedBioluminescent Bacteria for General Toxicity Assays.- InVivo Bioluminescent Imaging of ATP-BindingCassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of CancerGene Therapy.- Development of a Multicolor BioluminescenceImaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signalingand Apoptosis .- A Multichannel BioluminescenceDetermination platform for Bioassays.- A Bioluminescence Assay Systemfor Imaging Metal Cationic Activities in Urban Aerosols.- LuminescenceImaging: (a) Multi-color Visualization of Ca2+ Dynamics in DifferentCellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.- Photon Counting System for High-sensitivityDetection of Bioluminescence at Optical Fiber End.