Lipidomics

Häftad, Engelska, 2018

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This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes.

Introduction and Overview of Lipidomic Strategies.- Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry.- Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.- MALDI-Imaging Mass Spectrometry of Brain Lipids.- Ion-Mobility Mass Spectrometry for Lipidomics Applications.- In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteins: A Lesson from the Ceramide Transport Protein CERT.- Quantification of Endogenous Endocannabinoids by LC-MS/MS.- Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry.- Mass Spectrometric Analysis of Lipid Hydroperoxides.- Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradation of (2E)-hexadecenal in HepG2 Cell Lysates.- CE Analysis of Phospholipid Headgroups.- HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids.- Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures.- Ambient Lipidomic Analysis of Brain Tissue using Desorption Electrospray Ionization (DESI) Mass Spectrometry.- Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids.- Derivatization of Fatty Aldehydes and Ketones: Girard’s Reagent T (GRT).- Derivatization of Lipid Amines: Fluorenylmethyloxycarbonyl (FMOC).- High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products.