Mammalian Synthetic Promoters

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 484 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt över 249 kr.

Beskrivning

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application.

Produktinformation

Utgivningsdatum:2018-06-12
Mått:178 x 254 x 16 mm
Vikt:554 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:277
Förlag:Humana Press Inc.
ISBN:9781493984213

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Initial Considerations before Designing a Promoter Construct.- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter.- Secreted Reporters for Monitoring Multiple Promoter Function.- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo.- Monitoring Promoter Activity by Flow Cytometry.- Functional Screening of Core Promoter Activity.- Bioinformatically Informed Design of Synthetic Mammalian Promoters.- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication.- Constructing Strong Cell-Type-Specific Promoters through Informed Design.- PCR Assembly of Synthetic Promoters.- The Tetracycline Responsive System.- Light-responsive Promoters.- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation.- Promoter Activation with Electromagnetism.- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment.- A Modular Approach to Building Complex Synthetic Circuits.- Computational Sequence Design with R2oDNA Designer.- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells.