Host-Pathogen Interactions

Methods and Protocols

AvCarlos Medina,Francisco Javier López-Baena

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 167 kr

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Beskrivning

Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction.

Produktinformation

Utgivningsdatum:2018-05-23
Mått:178 x 254 x 19 mm
Vikt:655 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:317
Förlag:Humana Press Inc.
ISBN:9781493985326

Utforska kategorier

Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Genetic Association Studies in Host-Pathogens Interaction Analysis.- Bacterial Genotyping Methods: From the Basics to Modern.- Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection.- lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity.- Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage.- Organoids as a Model to Study Infectious Disease.- Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins.- Systems Biology Modeling to Study Pathogen-Host Interactions.- Phage Therapy: Various Perspectives on How to Improve the Art.- Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression.- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth.- Detection of Bacterial Quorum Sensing Molecules.- Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae.- Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation.- In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors.- Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains.- Methods to Quantify Biotic-Induced Stress in Plants.- From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy.- Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.- Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria.- A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean.- Methods for the Characterization of Plant-Growth Promoting Rhizobacteria.