Metabolomics

Methods and Protocols

AvSanjoy K. Bhattacharya

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

1 381 kr

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Beskrivning

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems.

Produktinformation

Utgivningsdatum:2020-08-14
Mått:178 x 254 x 20 mm
Vikt:663 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:335
Förlag:Humana Press Inc.
ISBN:9781493994908

Utforska kategorier

Innehållsförteckning

Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer.- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis.- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards.- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods.- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods.- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS.- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates.- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System.- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM.- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS).- Analysis of Microbial Siderophores by Mass Spectrometry.- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer.- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification.- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites.- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells.- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis.- Protocols for Metabolomic Analyses from Tissues in Parkinson’s Disease.- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease.- Proteometabolomics ofMelphalan Resistance in Multiple Myeloma.- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading.- Protocols for NMR Analysis in Livestock Metabolomics.- Metabolomics Applicable to Retinal Vascular Diseases.