Microglia

Methods and Protocols

AvOlga Garaschuk,Alexei Verkhratsky

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

1 314 kr

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Beskrivning

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades.

Produktinformation

Utgivningsdatum:2020-08-14
Mått:178 x 254 x 20 mm
Vikt:663 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:340
Förlag:Humana Press Inc.
ISBN:9781493996605

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Onkologi inom Medicin

Innehållsförteckning

Microglia: The Neural Cells of Non-Neural Origin.- Morphology of Microglia across Contexts of Health and Disease.- Physiology of Microglia.- Microglia Reactivity: Heterogeneous Pathological Phenotypes.- Microglia in Neurodegenerative Disorders.- Physiological Implications of Microglia-Synapse Interactions.- Ultrastructural Analyses of Microglial Interactions with Synapses.- Visualizing Dark Microglia.- Electrophysiological Investigation of Microglia.- Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia.- Assessment of Parasite-Microglia Interactions In Vitro.- In Vivo Visualization of Microglia Using Tomato Lectin.- Long-Term In Vivo Imaging of Individual Microglial Cells.- Analysis of Signaling Mechanisms Regulating Microglial Process Movement.- Measuring Microglial Turnover in the Adult Brain.- Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor.- Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia.- Labeling Microglia with Genetically-Encoded Calcium Indicators.- Calcium Imaging of Microglial Network Activity in Stroke.- In Vivo Two Photon Imaging of Microglial Synapse Contacts.- In Vivo Phagocytosis Analysis of Amyloid Beta.- Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models.- Analysis of the Microglial Sensome.- Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue.