Protein'Protein Interactions

From the reviews: "This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function "The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem "This ... volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. ... This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding ... ." (Wayne F. Patton, Proteomics, Issue 6, 2006)

• Overview.- Structural Basis of Protein-Protein Interactions.- Quantitative Analysis of Protein-Protein Interactions.- In Vitro Techniques.- Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry.- Circular Dichroism Analysis for Protein-Protein Interactions.- Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy.- Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance.- Using Light Scattering to Determine the Stoichiometry of Protein Complexes.- Sedimentation Equilibrium Studies.- Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography.- Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions.- Fluorescence Polarization Assay to Quantify Protein-Protein Interactions.- Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot.- Glutathione-S-Transferase-Fusion Based Assays for Studying Protein-Protein Interactions.- Affinity Capillary Electrophoresis Analyses of Protein—Protein Interactions in Target-Directed Drug Discovery.- Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting.- Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions.- Detecting Protein-Protein Interactions in Heterologous Systems.- A Bacterial Two-Hybrid System Based on Transcription Activation.- Using the Yeast Two-Hybrid System to Identify Interacting Proteins.- Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System.- The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System.- Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae.- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo.- Co-Immunoprecipitation from TransfectedCells.- Probing Protein-Protein Interactions in Living Cells.- Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET).- Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer.- Fluorescence Correlation Spectroscopy.- Confocal Microscopy for Intracellular Co-Localization of Proteins.- Mapping Biochemical Networks With Protein-Fragment Complementation Assays.- In Vivo Protein Cross-Linking.- Proteomics-Based Approaches.- Computational Prediction of Protein-Protein Interactions.- Affinity Methods for Phosphorylation-Dependent Interactions.- Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes.- Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry.- Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry.- Internet Resources for Studying Protein-Protein Interactions.