In Vitro Transcription and Translation Protocols

Inbunden, Engelska, 2007

Del i serien Methods in Molecular Biology

1 062 kr

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Beskrivning

This book is a highly anticipated update of the previous edition. It provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. It has been completely updated with new chapters and topics.

Produktinformation

Utgivningsdatum:2007-05-03
Mått:152 x 229 x 25 mm
Vikt:584 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:276
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781588295583

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik

Innehållsförteckning

Technologies.- Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions.- Continuous-Exchange Protein-Synthesizing Systems.- Cell-Free Production of Integral Membrane Proteins on a Preparative Scale.- SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression.- Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System.- Exogenous Protein Expression in Xenopus Oocytes.- Applications.- In Vitro Translation to Study HIV Protease Activity.- The Protein Truncation Test in Mutation Detection and Molecular Diagnosis.- Creation of Novel Enantioselective Lipases by SIMPLEX.- In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis.- In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation, and Immunoprecipitation Analysis.- Functional Expression of Type 1 Rat GABA Transporter in Microinjected Xenopus laevis Oocytes.- Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System.