Cardiovascular Disease, Volume 2

From the reviews: "Second volume ... in its 22 chapters describes methods for comprehension of disease mechanism at the molecular level. ... Each chapter contains detailed protocols needed for each practical step in course of experimentation so that it may serve as a guide even for beginners. ... intended for undergraduate and graduate students, postdoctoral fellows, clinicians and basic researchers, who are entering new areas of cardiovascular research and/or designing new experiments. Many protocols could be applied in research of another diseases as well." (Lubica Lacinova, General Physiology and Biophysics, January, 2007)

• Microarray Analysis of Cardiovascular Diseases.- Proteomics With Two-Dimensional Gel Electrophoresis and Mass Spectrometry Analysis in Cardiovascular Research.- Developing and Evaluating Genomics- or Proteomics-Based Diagnostic Tests.- Animal Models for Disease.- Generation of Transgenic Mice for Cardiovascular Research.- Quantitative Assay for Mouse Atherosclerosis in the Aortic Root.- Animal Models for Heart Failure.- Animal Models for Hypertension/Blood Pressure Recording.- Animal Models for Cardiac Arrhythmias.- Optical Mapping of Shock-Induced Arrhythmogenesis in the Rabbit Heart With Healed Myocardial Infarction.- Methods for Studying Voltage-Gated Sodium Channels in Heterologous Expression Systems.- Laser-Induced Thrombosis in Zebrafish Larvae.- Methods for Isolation of Endothelial and Smooth Muscle Cells and In Vitro Proliferation Assays.- Applications of Adenoviral Vector-Mediated Gene Transfer in Cardiovascular Research.- Murine and HIV-Based Retroviral Vectors for In Vitro and In Vivo Gene Transfer.- Efficient Transfection of Primary Cells Relevant for Cardiovascular Research by nucleofection®.- Cell Adhesion and Migration Assays.- Apoptosis Assays.- Preparation of Protein Crystals for X-Ray Structural Study.- Structural Elucidation of Integrin ?IIb?3 Cytoplasmic Domain by Nuclear Magnetic Resonance Spectroscopy.- Applications of Electron Cryo-Microscopy to Cardiovascular Research.- Stem Cells in Cardiovascular Disease.