Bacteriophages

Methods and Protocols, Volume 2: Molecular and Applied Aspects

AvMartha R. J. Clokie,Andrew Kropinski

Inbunden, Engelska, 2008

Del i serien Methods in Molecular Biology

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Beskrivning

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.

Produktinformation

Utgivningsdatum:2008-12-15
Mått:203 x 276 x 27 mm
Vikt:1 163 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:373
Upplaga:2009
Förlag:Humana Press Inc.
ISBN:9781603275644

Utforska kategorier

Innehållsförteckning

Bacteriophage Genomics.- Preparation of Bacteriophage Lysates and Pure DNA.- Approaches to the Compositional Analysis of DNA.- Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis.- Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing.- PCR and Partial Sequencing of Bacteriophage Genomes.- In Sffamily Identification of Genes in Bacteriophage DNA.- Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions.- In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators.- Molecular Phylogenetics: Testing Evolutionary Hypotheses.- Bacteriophage Transcriptomics and Proteomics.- Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2.- Quantification of Host and Phage mRNA Expression During Infection Using Real-Time PCR.- Oligonucleotide Microarrays for Bacteriophage Expression Studies.- Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles.- Phage Proteomics: Applications of Mass Spectrometry.- Community Bacteriophage Approaches.- Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinting Methods.- Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome.- Applied Aspects Of Bacteriophage Biology.- Phage Typing.- A Genetic Screen to Identify Bacteriophage Lysins.- General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein–Protein Interactions.- Isolation of Monoclonal Antibody Fragments from Phage Display Libraries.- Internet Resources of Interest to BacteriophageWorkers.