Cell Migration

Developmental Methods and Protocols

AvClaire M. Wells,Maddy Parsons

Inbunden, Engelska, 2011

Del i serien Methods in Molecular Biology

1 619 kr

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Beskrivning

Cell migration is a key component of many biological processes. This book provides a catalogue of techniques for the study of cell migration. It brings together a wide range of these techniques from the basic migration assays to recent technical advances.

Produktinformation

Utgivningsdatum:2011-07-15
Mått:178 x 254 x 29 mm
Vikt:1 085 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:463
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781617792069

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Cell Migration: An Overview.- Scratch-Wound Assay.- HGF-Induced DU145 Cell Scatter Assay.- Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time.- Imaging Cells Within 3D Cell Derived Matrix.- Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro.- Boyden Chamber.- Transwell® Invasion Assays.- Imaging Podosome Dynamics and Matrix Degradation.- Endothelial Cell Migration Under Flow.- In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.- Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy.- Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy.- Live Cell Fluorescence Microscopy Techniques.- Measuring Invasion in an Organotypic Model.- Analysis of Cell Migration Using Caenorhabditis elegans as a Model System.- Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration.- Measuring Inflammatory Cell Migration in the Zebrafish.- Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement.- Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants.- Simple Experimental and Spontaneous Metastasis Assays in Mice.- Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo.- Measuring Angiogenesis in Mice.- Time-Lapse Imaging of Chick Cardiac Precursor Cells.- Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy.- Fluorescence Recovery After Photobleaching.- Measuring FRET Using Time-Resolved FLIM.- Cell Migration in Confinement: A Micro-Channel Based Assay.-Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay.- Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays.