Claire M. Wells - Böcker

COMPREHENSIVE REFERENCE EXPLORING MICROSCOPY TECHNIQUES TO SUPPORT THE STUDY OF LIVING SYSTEMS WITH A UNIQUE MULTISCALE FOCUS Live Biological Imaging Across Scales presents an overview of the technologies and experimental strategies available for live imaging across scales. Each chapter highlights a different technique, working upwards from small to large scale. Readers will find critical evaluation of these techniques and perspectives on future developments in this evolving field, enabling them to effectively identify techniques that may benefit their research activities. Written by a team of leading experts and part of the Wiley-Royal Microscopical Society series, Live Biological Imaging Across Scales includes information on: Single cells, covering single molecule tracking/FRET, actin, microtubules, intermediate filaments, septins, vesicles, phagosomes, and confined environmentsMultiple cells, covering collective migration, endothelial monolayers, flow platelets, and flow, wound healing, monocyte transmigration, cancer spheroids/tumor models, and organoidsOrganisms, covering Dictyostelium, C. elegans, Drosophila, and mouse-tumorsImaging of whole cells individually and collectively in both 2D and 3D platformsLive Biological Imaging Across Scales is an essential reference for microscopists and experimental biologists, particularly those interested and/or working in the expanding field of live imaging in biology, as well as those working in the commercial microscopy industry supply and development.

Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study.  The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances.  Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D.  It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for anyresearcher who wishes to explore this significant area of cell biology.