Barry Mendelow is Emeritus Professor at the University of the Witwatersrand and National Health Laboratory Service, was formerly the founding Professor and Head of the Division of Molecular Medicine and Haematology at the School of Pathology.Michèle Ramsay is Professor and Head of the Molecular Genetics Laboratory at the University of the Witwatersrand, Johannesburg.Nanthakumarn Chetty is Associate Professor and head of the Platelet Research Unit in the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.Wendy Stevens is Head of the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.

• Foreword Acknowledgements Chapter 1 Introduction Barry V Mendelow and Penny KeeneKEYNOTE ESSAY 1: Defining Who We Are: DNA in Forensics, Genealogy and Human OriginsHimla SoodyallSECTION 1 PRINCIPLES OF CELLULAR AND 13MOLECULAR BIOLOGYChapter 2 Digital Concepts in Molecular Medicine Barry V MendelowChapter 3 The Anatomy and Physiology of the Genome Marc S Weinberg, Natalie A Whalley and Michèle RamsayChapter 4 Molecular Cell BiologyNatalie A Whalley, Sarah Walters and Kate HammondChapter 5 Genetic Variation Silke Arndt and Anthony B LaneChapter 6 Genes in Development Lillian A Ouko and Michèle RamsayChapter 7 Tools of Molecular Medicine (a) Nucleic Acid Methods Natela Rekhviashvili and Gwynneth Stevens(b) Protein Methods Alexio Capovilla(c) Cellular Phenotyping and Flow Cytometry Lesley E Scott and Debbie K Glencross(d) Molecular Cytogenetics Pascale Willem and Jacqueline BrownKEYNOTE ESSAY 2: The Human Genome Michèle RamsaySECTION 2 MOLECULAR PATHOLOGY Chapter 8 Genomes and the Environment:An Overview of Molecular PathologyBarry V MendelowChapter 9 Genetics, Genomics, Health and Disease: General Considerations Arnold L ChristiansonChapter 10 Chromosome Disorders Sarah Walters and Nerine E GregersenChapter 11 Mendelian Inheritance Anthony B LaneChapter 12 Unusual Molecular Processes that Impact on Disease Tabitha Haw and Amanda KrauseChapter 13 Population Genetics Anthony B Lane and Himla SoodyallChapter 14 Complex Multifactorial Inheritance Zané LombardMolecular MedicineChapter 15 Molecular Basis for Phenotypic Variation Nerine E Gregersen and Amanda KrauseChapter 16 Medical Genetics Nerine E Gregersen and Amanda KrauseKEYNOTE ESSAY 3: Human Cloning: Should We Go There? Barry V MendelowChapter 17 Neoplasia: General Considerations Alan C Paterson and Leandra CronjéChapter 18 Oncogenes Natalie A Whalley and Kate HammondChapter 19 Mammalian DNA Repair Alexio CapovillaChapter 20 Tumour Suppressor Genes and Inherited Susceptibility to Cancer Kate HammondChapter 21 Carcinoma Alan C Paterson and Leandra CronjéChapter 22 Leukaemias and Lymphomas Tracey M Wiggill, Nicole S Holland, Pascale Willem and Lindsay EarlamChapter 23 Molecular Approaches to the Diagnosis, Prognostication and Monitoring of CancerWendy StevensKEYNOTE ESSAY 4: Microbes, Molecules, Maladies and Man Adriano G DuseChapter 24 Molecular Basis of Infectious Diseases: General Considerations Wendy StevensChapter 25 Immunology Wolfgang Prinz, Mieneke Smit van Dixhoorn, Elizabeth Mayne andAhmed A WadeeChapter 26 Human Immunodeficiency Virus Wendy Stevens and Maria A PapathanasopoulosChapter 27 Tuberculosis Gerrit Coetzee and Hendrik J KoornhofChapter 28 Malaria Theresa L CoetzerChapter 29 Influenza Barry D SchoubChapter 30 Oncogenic Viruses Patrick ArbuthnotChapter 31 Vaccines and Immunisation Maria A PapathanasopoulosKEYNOTE ESSAY 5: Drugs and the 21st Century Nicole HollandSECTION 3 MOLECULAR THERAPEUTICS Chapter 32 Targets for Molecular Therapy: The Biology of HaemostasisMarion Münster and Nanthakumarn ChettyChapter 33 Cellular Targets of Antiplatelet Agents Nanthakumarn Chetty and Marion MünsterChapter 34 Rational Drug Design Grant B NapierChapter 35 Chronic Myeloid Leukaemia Nerina SavageChapter 36 Gene Therapy Marc S Weinberg and Patrick ArbuthnotChapter 37 Diabetes Mellitus Nigel J CrowtherChapter 38 Pharmacogenetics Chrisna Durandt, Sahle M Asfaha and Michael S PepperChapter 39 Basic Molecular Biology of Blood Groups Wolfgang PrinzKEYNOTE ESSAY 6: Molecular Research Case Study:Developing Novel RNA Interference-based TherapyPatrick Arbuthnot and Marc S WeinbergSECTION 4 RESEARCH AND THE CONTINUING EVOLUTION OF MOLECULAR MEDICINEChapter 40 Approaches to Research in Molecular Medicine Michèle RamsayChapter 41 Bioinformatics and Molecular Medicine Andries J Oelofse and Michèle RamsayChapter 42 Personalised Medicine: Dream or Reality? Wendy StevensGLOSSARY CONTRIBUTORS’ BIOGRAPHIESSOURCE MATERIAL AND RECOMMENDED READING PERMISSIONS AND CREDITS INDEX

Barry Mendelow is Emeritus Professor at the University of the Witwatersrand and National Health Laboratory Service, was formerly the founding Professor and Head of the Division of Molecular Medicine and Haematology at the School of Pathology.Michèle Ramsay is Professor and Head of the Molecular Genetics Laboratory at the University of the Witwatersrand, Johannesburg.Nanthakumarn Chetty is Associate Professor and head of the Platelet Research Unit in the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.Wendy Stevens is Head of the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.

• Foreword Acknowledgements Chapter 1 Introduction Barry V Mendelow and Penny KeeneKEYNOTE ESSAY 1: Defining Who We Are: DNA in Forensics, Genealogy and Human OriginsHimla SoodyallSECTION 1 PRINCIPLES OF CELLULAR AND 13MOLECULAR BIOLOGYChapter 2 Digital Concepts in Molecular Medicine Barry V MendelowChapter 3 The Anatomy and Physiology of the Genome Marc S Weinberg, Natalie A Whalley and Michèle RamsayChapter 4 Molecular Cell BiologyNatalie A Whalley, Sarah Walters and Kate HammondChapter 5 Genetic Variation Silke Arndt and Anthony B LaneChapter 6 Genes in Development Lillian A Ouko and Michèle RamsayChapter 7 Tools of Molecular Medicine (a) Nucleic Acid Methods Natela Rekhviashvili and Gwynneth Stevens(b) Protein Methods Alexio Capovilla(c) Cellular Phenotyping and Flow Cytometry Lesley E Scott and Debbie K Glencross(d) Molecular Cytogenetics Pascale Willem and Jacqueline BrownKEYNOTE ESSAY 2: The Human Genome Michèle RamsaySECTION 2 MOLECULAR PATHOLOGY Chapter 8 Genomes and the Environment:An Overview of Molecular PathologyBarry V MendelowChapter 9 Genetics, Genomics, Health and Disease: General Considerations Arnold L ChristiansonChapter 10 Chromosome Disorders Sarah Walters and Nerine E GregersenChapter 11 Mendelian Inheritance Anthony B LaneChapter 12 Unusual Molecular Processes that Impact on Disease Tabitha Haw and Amanda KrauseChapter 13 Population Genetics Anthony B Lane and Himla SoodyallChapter 14 Complex Multifactorial Inheritance Zané LombardMolecular MedicineChapter 15 Molecular Basis for Phenotypic Variation Nerine E Gregersen and Amanda KrauseChapter 16 Medical Genetics Nerine E Gregersen and Amanda KrauseKEYNOTE ESSAY 3: Human Cloning: Should We Go There? Barry V MendelowChapter 17 Neoplasia: General Considerations Alan C Paterson and Leandra CronjéChapter 18 Oncogenes Natalie A Whalley and Kate HammondChapter 19 Mammalian DNA Repair Alexio CapovillaChapter 20 Tumour Suppressor Genes and Inherited Susceptibility to Cancer Kate HammondChapter 21 Carcinoma Alan C Paterson and Leandra CronjéChapter 22 Leukaemias and Lymphomas Tracey M Wiggill, Nicole S Holland, Pascale Willem and Lindsay EarlamChapter 23 Molecular Approaches to the Diagnosis, Prognostication and Monitoring of CancerWendy StevensKEYNOTE ESSAY 4: Microbes, Molecules, Maladies and Man Adriano G DuseChapter 24 Molecular Basis of Infectious Diseases: General Considerations Wendy StevensChapter 25 Immunology Wolfgang Prinz, Mieneke Smit van Dixhoorn, Elizabeth Mayne andAhmed A WadeeChapter 26 Human Immunodeficiency Virus Wendy Stevens and Maria A PapathanasopoulosChapter 27 Tuberculosis Gerrit Coetzee and Hendrik J KoornhofChapter 28 Malaria Theresa L CoetzerChapter 29 Influenza Barry D SchoubChapter 30 Oncogenic Viruses Patrick ArbuthnotChapter 31 Vaccines and Immunisation Maria A PapathanasopoulosKEYNOTE ESSAY 5: Drugs and the 21st Century Nicole HollandSECTION 3 MOLECULAR THERAPEUTICS Chapter 32 Targets for Molecular Therapy: The Biology of HaemostasisMarion Münster and Nanthakumarn ChettyChapter 33 Cellular Targets of Antiplatelet Agents Nanthakumarn Chetty and Marion MünsterChapter 34 Rational Drug Design Grant B NapierChapter 35 Chronic Myeloid Leukaemia Nerina SavageChapter 36 Gene Therapy Marc S Weinberg and Patrick ArbuthnotChapter 37 Diabetes Mellitus Nigel J CrowtherChapter 38 Pharmacogenetics Chrisna Durandt, Sahle M Asfaha and Michael S PepperChapter 39 Basic Molecular Biology of Blood Groups Wolfgang PrinzKEYNOTE ESSAY 6: Molecular Research Case Study:Developing Novel RNA Interference-based TherapyPatrick Arbuthnot and Marc S WeinbergSECTION 4 RESEARCH AND THE CONTINUING EVOLUTION OF MOLECULAR MEDICINEChapter 40 Approaches to Research in Molecular Medicine Michèle RamsayChapter 41 Bioinformatics and Molecular Medicine Andries J Oelofse and Michèle RamsayChapter 42 Personalised Medicine: Dream or Reality? Wendy StevensGLOSSARY CONTRIBUTORS’ BIOGRAPHIESSOURCE MATERIAL AND RECOMMENDED READING PERMISSIONS AND CREDITS INDEX

Barry Mendelow is Emeritus Professor at the University of the Witwatersrand and National Health Laboratory Service, was formerly the founding Professor and Head of the Division of Molecular Medicine and Haematology at the School of Pathology.Michèle Ramsay is Professor and Head of the Molecular Genetics Laboratory at the University of the Witwatersrand, Johannesburg.Nanthakumarn Chetty is Associate Professor and head of the Platelet Research Unit in the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.Wendy Stevens is Head of the Division of Molecular Medicine and Haematology at the University of the Witwatersrand, Johannesburg.

• Foreword Acknowledgements Chapter 1 Introduction Barry V Mendelow and Penny KeeneKEYNOTE ESSAY 1: Defining Who We Are: DNA in Forensics, Genealogy and Human OriginsHimla SoodyallSECTION 1 PRINCIPLES OF CELLULAR AND 13MOLECULAR BIOLOGYChapter 2 Digital Concepts in Molecular Medicine Barry V MendelowChapter 3 The Anatomy and Physiology of the Genome Marc S Weinberg, Natalie A Whalley and Michèle RamsayChapter 4 Molecular Cell BiologyNatalie A Whalley, Sarah Walters and Kate HammondChapter 5 Genetic Variation Silke Arndt and Anthony B LaneChapter 6 Genes in Development Lillian A Ouko and Michèle RamsayChapter 7 Tools of Molecular Medicine (a) Nucleic Acid Methods Natela Rekhviashvili and Gwynneth Stevens(b) Protein Methods Alexio Capovilla(c) Cellular Phenotyping and Flow Cytometry Lesley E Scott and Debbie K Glencross(d) Molecular Cytogenetics Pascale Willem and Jacqueline BrownKEYNOTE ESSAY 2: The Human Genome Michèle RamsaySECTION 2 MOLECULAR PATHOLOGY Chapter 8 Genomes and the Environment:An Overview of Molecular PathologyBarry V MendelowChapter 9 Genetics, Genomics, Health and Disease: General Considerations Arnold L ChristiansonChapter 10 Chromosome Disorders Sarah Walters and Nerine E GregersenChapter 11 Mendelian Inheritance Anthony B LaneChapter 12 Unusual Molecular Processes that Impact on Disease Tabitha Haw and Amanda KrauseChapter 13 Population Genetics Anthony B Lane and Himla SoodyallChapter 14 Complex Multifactorial Inheritance Zané LombardMolecular MedicineChapter 15 Molecular Basis for Phenotypic Variation Nerine E Gregersen and Amanda KrauseChapter 16 Medical Genetics Nerine E Gregersen and Amanda KrauseKEYNOTE ESSAY 3: Human Cloning: Should We Go There? Barry V MendelowChapter 17 Neoplasia: General Considerations Alan C Paterson and Leandra CronjéChapter 18 Oncogenes Natalie A Whalley and Kate HammondChapter 19 Mammalian DNA Repair Alexio CapovillaChapter 20 Tumour Suppressor Genes and Inherited Susceptibility to Cancer Kate HammondChapter 21 Carcinoma Alan C Paterson and Leandra CronjéChapter 22 Leukaemias and Lymphomas Tracey M Wiggill, Nicole S Holland, Pascale Willem and Lindsay EarlamChapter 23 Molecular Approaches to the Diagnosis, Prognostication and Monitoring of CancerWendy StevensKEYNOTE ESSAY 4: Microbes, Molecules, Maladies and Man Adriano G DuseChapter 24 Molecular Basis of Infectious Diseases: General Considerations Wendy StevensChapter 25 Immunology Wolfgang Prinz, Mieneke Smit van Dixhoorn, Elizabeth Mayne andAhmed A WadeeChapter 26 Human Immunodeficiency Virus Wendy Stevens and Maria A PapathanasopoulosChapter 27 Tuberculosis Gerrit Coetzee and Hendrik J KoornhofChapter 28 Malaria Theresa L CoetzerChapter 29 Influenza Barry D SchoubChapter 30 Oncogenic Viruses Patrick ArbuthnotChapter 31 Vaccines and Immunisation Maria A PapathanasopoulosKEYNOTE ESSAY 5: Drugs and the 21st Century Nicole HollandSECTION 3 MOLECULAR THERAPEUTICS Chapter 32 Targets for Molecular Therapy: The Biology of HaemostasisMarion Münster and Nanthakumarn ChettyChapter 33 Cellular Targets of Antiplatelet Agents Nanthakumarn Chetty and Marion MünsterChapter 34 Rational Drug Design Grant B NapierChapter 35 Chronic Myeloid Leukaemia Nerina SavageChapter 36 Gene Therapy Marc S Weinberg and Patrick ArbuthnotChapter 37 Diabetes Mellitus Nigel J CrowtherChapter 38 Pharmacogenetics Chrisna Durandt, Sahle M Asfaha and Michael S PepperChapter 39 Basic Molecular Biology of Blood Groups Wolfgang PrinzKEYNOTE ESSAY 6: Molecular Research Case Study:Developing Novel RNA Interference-based TherapyPatrick Arbuthnot and Marc S WeinbergSECTION 4 RESEARCH AND THE CONTINUING EVOLUTION OF MOLECULAR MEDICINEChapter 40 Approaches to Research in Molecular Medicine Michèle RamsayChapter 41 Bioinformatics and Molecular Medicine Andries J Oelofse and Michèle RamsayChapter 42 Personalised Medicine: Dream or Reality? Wendy StevensGLOSSARY CONTRIBUTORS’ BIOGRAPHIESSOURCE MATERIAL AND RECOMMENDED READING PERMISSIONS AND CREDITS INDEX