This book LNCS 15681 constitutes the refereed proceedings of the 24th International Conference on Mathematical Optimization Theory and Operations Research, MOTOR 2025, held in Novosibirsk, Russia, during July 7–11, 2025.The 27 full papers were carefully reviewed and selected from 72 submissions. The proceeding focus on Mathematical Programming; Optimal Control; Game Theory; Operations Research and Applications; Machine Learning and Optimization.