This book constitutes the refereed proceedings of the 5th EAI International Conference on Technology, Innovation, Entrepreneurship and Education, TIE 2025, held in Cambridge, UK, during March 17-18, 2025.The 8 full papers included in this book were carefully reviewed and selected from 29 submissions. These papers provide a vision of technologies applied to enterprise, education, medicine and cultural heritage, reflecting our collective ambition to explore pioneering ideas and redefine the future of learning, innovation, and societal progress.