This volume constitutes the revised selected papers of 18th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2025, in Poto, Portugal, during February 20–22, 2025.The 25 full papers and 11 short papers presented in this volume were carefully reviewed and selected from 299 submissions. They are grouped into the following topics: Biomedical Electronics and Devices; Bioimaging; Bioinformatics Models, Methods and Algorithms; Bio-inspired Systems and Signal Processing; Health Informatics.