Plant Microtechniques and Protocols

Edward C. YeungDepartment of Biological Sciences, University of Calgary, Calgary, Alberta, CanadaClaudio StasollaDepartment of Plant Science, University of Manitoba, Winnipeg, Manitoba, CanadaMichael J. SumnerDepartment of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, CanadaBing Q. HuangMayo Clinic, Rochester, Minnesota, USA

• A Guide to the Study of Plant Structure with Emphasis on Living Specimens.- Chemical and Physical Fixation of Cells and Tissues: an Overview.- Paraffin and Polyester Waxes.- The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100.- Epoxy Resins for Light and Transmission Electron Microscopy.- LR White Acrylic Resin.- High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples.- Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens.- Fluorescent Staining of Living Plant Cells.- Improved Methods for Clearing and Staining of Plant Samples.- Whole Mount Immunofluorescence Staining of Plant Cells and Tissues.- Protoplast Isolation and Staining.- Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving.- Abscisic Acid Immunostaining.- Plant Chromosome Preparations and Staining for Light Microscopic Studies.- Chromosome techniques and FISH.- Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2’-deoxyuridine (EdU).- Staining Methods for Programmed Cell Death.- Laser Microdissection of Plant Tissues.-RNA In situ Hybridization.- Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy.- Collecting and Processing Wood Microcores for Monitoring Xylogenesis.- Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy.- Collection of Plant Remains from Archaeological Contexts.- Archaeological Wood Preparation.- Archaeopalynological Preparation Techniques.- Phytoliths: Preparation and Archaeological Extraction.- Starch Granules: Preparation and Archaeological Extraction.- Plant Collection, Identification, and Herbarium Procedures.- Index.