Plant Microtechniques and Protocols

AvEdward Chee Tak Yeung,Claudio Stasolla

Häftad, Engelska, 2016

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Beskrivning

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.

Produktinformation

Utgivningsdatum:2016-10-29
Mått:155 x 235 x 32 mm
Vikt:885 g
Format:Häftad
Språk:Engelska
Antal sidor:576
Förlag:Springer International Publishing AG
ISBN:9783319359359

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik

Mer om författaren

Edward C. YeungDepartment of Biological Sciences, University of Calgary, Calgary, Alberta, CanadaClaudio StasollaDepartment of Plant Science, University of Manitoba, Winnipeg, Manitoba, CanadaMichael J. SumnerDepartment of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, CanadaBing Q. HuangMayo Clinic, Rochester, Minnesota, USA

Innehållsförteckning

A Guide to the Study of Plant Structure with Emphasis on Living Specimens.- Chemical and Physical Fixation of Cells and Tissues: an Overview.- Paraffin and Polyester Waxes.- The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100.- Epoxy Resins for Light and Transmission Electron Microscopy.- LR White Acrylic Resin.- High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples.- Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens.- Fluorescent Staining of Living Plant Cells.- Improved Methods for Clearing and Staining of Plant Samples.- Whole Mount Immunofluorescence Staining of Plant Cells and Tissues.- Protoplast Isolation and Staining.- Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving.- Abscisic Acid Immunostaining.- Plant Chromosome Preparations and Staining for Light Microscopic Studies.- Chromosome techniques and FISH.- Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2’-deoxyuridine (EdU).- Staining Methods for Programmed Cell Death.- Laser Microdissection of Plant Tissues.-RNA In situ Hybridization.- Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy.- Collecting and Processing Wood Microcores for Monitoring Xylogenesis.- Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy.- Collection of Plant Remains from Archaeological Contexts.- Archaeological Wood Preparation.- Archaeopalynological Preparation Techniques.- Phytoliths: Preparation and Archaeological Extraction.- Starch Granules: Preparation and Archaeological Extraction.- Plant Collection, Identification, and Herbarium Procedures.- Index.