Tumor Microenvironment

Study Protocols

AvConstantinos Koumenis,Lisa M. Coussens

Häftad, Engelska, 2018

Del 899 i serien Advances in Experimental Medicine and Biology

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Beskrivning

This volume covers the topics presented at the 3rd International Conference on Tumor Microenvironment and Cellular Stress by an international community of researchers.

Produktinformation

Utgivningsdatum:2018-05-30
Mått:155 x 235 x 17 mm
Vikt:470 g
Format:Häftad
Språk:Engelska
Serie:Advances in Experimental Medicine and Biology
Antal sidor:297
Förlag:Springer International Publishing AG
ISBN:9783319799933

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Innehållsförteckning

1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells.- 2.Measuring DNA replication rates in hypoxic conditions.- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions.- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis.- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells.- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy.- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells.- 8.Measuring Autophagy in the Context of Cancer.- 9.Methods for studying autophagy within the tumor microenvironment.- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia.- 11.Isolation of Glioma Initiating Cells for Biological Study.- 12.Isolation of mouse and human tumor associated macrophages.- 13.Mouse models of brain metastasis for unravelling tumour progression.- 14.Quantification of lung metastases from in vivo mouse models.- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA).- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.