Analyzing Microbes

From the reviews: "The Analyzing Microbes--Manual of Molecular Biology Techniques is indeed a practical guide to the application of various molecular biology techniques in general microbiological research, with special emphasis in/on biotechnology and environmental microbiology. ... All chapters are written in a concise step-by-step algorithm and are easy to follow during reading or bench working. ... In my opinion, the aim of this book ... to produce a self-contained laboratory manual which will be useful to both experienced practitioners and beginners in the field, is fully accomplished." (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)

• Microbial DNA extraction, purification and quantitation.- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR.- Restriction enzymes and their role in microbiology.- Genetic fingerprinting techniques  for molecular characterization of microbes.- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles.- Molecular identification of microbes.- Preservation and maintenance of microbial cultures.- Microbes from extreme environment: Molecular identification procedures.- ELISA based identification and detection of microbes.- Analysis of microbial diversity and construction of metagenomic library.- Bioinformatics tools for interpretation of data used in molecular identification.- Molecular phylogenetics of microbes.- Microarray technology: Basic concept, protocols and applications.- Microarray analysis of different functional genes of microorganisms.- DNA cloning and sequencing.- Biological sequence analysis: Algorithms and statistical methods.