Molecular Markers and Plant Biotechnology

AvRukam S. Tomar,Manoj V. Parakhia

Häftad, Engelska, 2010

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Beskrivning

The book Molecular Markers and Plant Biotechnology is an indispensable resource for those in the field of biotechnology. This comprehensive collection of molecular marker-based techniques is presented in 40 chapters and includes 578 pages of in-depth information, as well as numerous figures to aid in understanding. The book also provides practical information about relevant equipment and laboratory safety rules, which are essential for safe and effective work. The appendices include basic calculations and principles in the preparation of reagents, as well as a glossary and abbreviations to ensure accuracy. This text is an essential resource for any biotechnology laboratory or class.

Produktinformation

Utgivningsdatum:2010-01-15
Mått:42 x 152 x 229 mm
Vikt:1 390 g
Format:Häftad
Språk:Engelska
Antal sidor:176
Förlag:New India Publishing Agency
ISBN:9788119235230

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Mer om författaren

Rukam S. Tomar: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. ProfessorManoj V. Parakhia:Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, ProfessorSunil V.Patel: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor.B.A. Golakiya: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat.

Innehållsförteckning

1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis (PAGE). 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers (PCR Machines). 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA (RAPD). 16. Restriction Fragment Length Polymorphism (RFLP). 17. Amplified Fragment Length Polymorphism (AFLP). 18. Simple sequence repeats (Microsatellite). 19. Variable number of Tandem repeats (Minisatellite). 20. Inter Simple Sequence Repeat (ISSR). 21. Sequence Characterized Amplified Region (SCAR). 22. Cleaved Amplified Polymorphic Sequence (CAPS). 23. Single-Strand Conformation Polymorphism (SSCP). 24. Retrotransposon-based markers (S-SAP, IRAP, REMAP, RBIP, RGAS, SNP). 25. Silver staining. 26. Basics of marker assisted selection in crop plants.27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization (FISH). 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.