Dr. H.P. Puttaraju, M.Sc., Ph.D., FRES is a Professor of Sericulture/ Life Sciences and Co-ordinator, Biological Sciences (Integrated M.Sc.) of Bangalore University, Bangalore, Karnataka. With acquiring advanced training at Cardiff University of Wales, UK, he has investigated and studied the importance of Wolbachia endosymbiont in insect and insect pests using molecular techniques. Using DNA markers he and his group have analyzed several plant and insect genotypes. So far he has successfully guided 11 Ph.D and 7 M. Phil students on various aspects of Life Sciences. He has authored/edited 2 books in his field of interest and published over 70 research papers in reputed national and international journals. He is recipient of Sir C.V. Raman Young Scientist Karnataka State Award, Young Histochemist Award, and is a Fellow of Royal Entomological Society, Fellow of Commonwealth Academic Staff Scholarship Commission, UK visiting fellow under INSA-Royal Society bilateral exchange programme.Dr. B.M. Prakash, M.Sc., Ph.D. has completed his doctoral studies with Seri-Biotechnology as the field specialization at Bangalore University and joined JNCASR for post doctoral research. His research in Ph.D programme includes understanding the infection frequency of Wolbachia (vertically transmitted bacterial endosymbiont) in insect pests associated with sericulture and its possible role in controlling uzifly, Exorista sorbillans, a serious tachinid endoparasitoid of silkworm, Bombyx mori L.Mr. B.C. Keshava Murthy, M.Sc., Ph.D. has completed in Ph.D. degree very recently with specialization in Plant Biotechnology. He joined MAS Lab. Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bangalore for Post Doctoral Research. His area of interest lies in application of DNA markers in Plants with particular references to Molecular Breeding and Genome Mapping.

• 1.Laboratory Equipments and Its Usage 2.Buffers 3. Cell Culture 15-45 4. Nucleic Acid Extraction 47-64 5. Determination of Quality and Quantity ofNucleic Acids 65-70 6. Polymerase Chain Reaction 71-83 7. Electrophoresis 85-100 8. Restriction Digestion of Enzymes 101-108 9. Blotting 109-132 10. Cloning 133-143 11. Sequencing of Nucleic Acids 145-157

Dr. H.P. Puttaraju, M.Sc., Ph.D., FRES is a Professor of Sericulture/ Life Sciences and Co-ordinator, Biological Sciences (Integrated M.Sc.) of Bangalore University, Bangalore, Karnataka. With acquiring advanced training at Cardiff University of Wales, UK, he has investigated and studied the importance of Wolbachia endosymbiont in insect and insect pests using molecular techniques. Using DNA markers he and his group have analyzed several plant and insect genotypes. So far he has successfully guided 11 Ph.D and 7 M. Phil students on various aspects of Life Sciences. He has authored/edited 2 books in his field of interest and published over 70 research papers in reputed national and international journals. He is recipient of Sir C.V. Raman Young Scientist Karnataka State Award, Young Histochemist Award, and is a Fellow of Royal Entomological Society, Fellow of Commonwealth Academic Staff Scholarship Commission, UK visiting fellow under INSA-Royal Society bilateral exchange programme.Dr. B.M. Prakash, M.Sc., Ph.D. has completed his doctoral studies with Seri-Biotechnology as the field specialization at Bangalore University and joined JNCASR for post doctoral research. His research in Ph.D programme includes understanding the infection frequency of Wolbachia (vertically transmitted bacterial endosymbiont) in insect pests associated with sericulture and its possible role in controlling uzifly, Exorista sorbillans, a serious tachinid endoparasitoid of silkworm, Bombyx mori L.Mr. B.C. Keshava Murthy, M.Sc., Ph.D. has completed in Ph.D. degree very recently with specialization in Plant Biotechnology. He joined MAS Lab. Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bangalore for Post Doctoral Research. His area of interest lies in application of DNA markers in Plants with particular references to Molecular Breeding and Genome Mapping.

• 1.Laboratory Equipments and Its Usage 2.Buffers 3. Cell Culture 15-45 4. Nucleic Acid Extraction 47-64 5. Determination of Quality and Quantity ofNucleic Acids 65-70 6. Polymerase Chain Reaction 71-83 7. Electrophoresis 85-100 8. Restriction Digestion of Enzymes 101-108 9. Blotting 109-132 10. Cloning 133-143 11. Sequencing of Nucleic Acids 145-157