Biomedical Light Microscopy

AvJ. James,H.J Tanke

Häftad, Engelska, 2012

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Beskrivning

New interest in light microscopy of the last few years has not been backed up by adequate general literature.

Produktinformation

Utgivningsdatum:2012-10-04
Mått:155 x 235 x 12 mm
Vikt:324 g
Format:Häftad
Språk:Engelska
Antal sidor:192
Förlag:Springer
ISBN:9789401056823

Utforska kategorier

Anatomi inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin
Zoologi inom Naturvetenskap och teknik

Innehållsförteckning

1. Light microscopy as an optical system, the stand and its parts.- 1.1 Basic theory.- 1.2 The objective as an optical tool; resolving power.- 1.3 Eyepieces.- 1.4 Objective and eyepiece as an integrated system.- Recommended further reading.- 2. The light microscope as a tool for observation and measurement: illumination and image formation.- 2.1 Modulation of the illuminating light by the object.- 2.2 The stand and its parts.- 2.3 Illumination and image formation.- Recommended further reading.- 3. Fluorescence microscopy.- 3.1 Theoretical background.- 3.2 The fluorescence microscope.- Recommended further reading.- 4. Special optical techniques of image formation.- 4.1 Phase-contrast microscopy.- 4.2 Interferometry and interference contrast.- 4.3 Modulation-contrast microscopy.- 4.4 Polarization microscopy.- 4.5 Reflection microscopy and reflection-contrast microscopy.- 4.6 Acoustic microscopy.- 4.7 Superresolution: modern developments.- Recommended further reading.- 5. Reproduction of microscopic images, microphotography.- 5.1 Drawing and drawing apparatuses.- 5.2 Microprojection.- 5.3 Television microscopy.- 5.4 Photomicrography.- Recommended further reading.- 6. Quantitative analysis of microscopic images.- 6.1 Introduction.- 6.2 Morphometric techniques.- 6.3 Counting methods.- 6.4 Absorption and fluorescence measurement of cells.- 6.5 Absorption cytophotometry (cytophotometry or microphotometry).- 6.6 Fluorescence cytophotometry (cytofluorometry, microfluorometry).- 6.7 Flow cytometry.- 6.8 Microspectrophotometry.- Recommended further reading.- 7. Automation: image analysis and pattern recognition.- 7.1 General introduction.- 7.2 Scanning of microscopic objects: special cameras.- 7.3 The digitized image.- 7.4 Image analysis.- 7.5 Pattern recognition.- Recommended further reading.- 8. Appendix: technical aspects of the microscopical observation in practice.- 8.1 Introduction.- 8.2 Setting up a microscope for Köhler illumination.- 8.3 Again: the object.- 8.4 On the way through the object.- 8.5 Maintenance and minor technical problems.- 8.6 Frequently occurring minor defects.- Recommended further reading.- Index of subjects.