This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Introduction.- Analysis of GAPDH enzyme activity: A quantitative and qualitative approach.- Analysis of GAPDH - Native protein.- Analysis of GAPDH under Denaturing Conditions.- Analysis of GAPDH and Protein Interaction.- Analysis of GAPDH and Nucleic acid Interaction.- Analysis of Subcellular and Extracellular GAPDH.- Analysis of GAPDH posttranslational modifications.- Analysis of GAPDH by Mass Spectrometry.- Purification of GAPDH.