Advanced Imaging and Bio Techniques for Convergence Science

1. Jun Ki Kim: Asan Institute for Life Sciences, Asan Medical Center, Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, Korea (Republic of) kim@amc.seoul.kr2. Jeong Kon Kim: Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (Republic of) kim.jeongkon@gmail.com3. Chan-Gi Pack: Asan Institute for Life Sciences, Asan Medical Center, Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, Korea (Republic of) changipack@amc.seoul.kr

• 1.Confocal laser scanning microscopy and fluorescence correlation methods for evaluation of molecular interactions.- 2.Number and Brightness Imaging: visualization of protein oligomerization state in living cell.- 3.Single-molecule analysis on the coupling of molecular state and reaction kinetics.- 4.Imaging lipid rafts in the plasma membrane using novel probe and super-resolution microscopy.- 5.Multiplex Immunohistochemistry (IHC).- 6.Micro-endoscopy for live small animal fluorescent imaging.- 7.Immuno-gold techniques.- 8.Correlative Light and Electron Microscopy for Nanoparticle-Cell Interaction.- 9.Diagnostic and Therapeutic Nanomedicine.- 10.Semiconductor nanocrystals for biological imaging and fluorescence spectroscopy.- 11.DNA/RNA fluorescence imaging by synthetic nucleic acids.- Section II: Label-free techniques and advanced tomographic imaging.- 12.“Magnetic resonance imaging for preclinical study.- 13.Holotomography techniques for 3D label-free imaging of live cells and tissues.- 14.Dynamic-contrast optical imaging techniques.- 15.Emerging biological and clinical applications of photoacoustic tomography.- Section III: Potential (synergic) biotechnologies to be joined with imaging techniques.- 16.Generation of genetically-engineered mouse models using CRISPR-Cas system.- 17.Highly efficient generation of cell lines stably expressing genes of interest via Cre recombinase mediated knock-in method in mouse embryonic stem (ES) cells and its application for microscopic imaging techniques.- 18.Mass spectrometry based metabolomics in translational research.- 19.Application of mass spectrometry in drug metabolism and pharmacokinetics for drug development.- 20.Discovery and Application of Chemical Probes for Translational Research.- 21.Regeneration of skin cells by mesenchymal stem cell-derived exosomes.