A. Sankara Narayanan – författare

This volume provides lab-oriented protocols to deal the various plant microbiome engineering approaches in a lucid and simple manner.  Chapters are divided into four section detailing plant associated microbiomes, single cell genomics, whole community metagenomics, metabolic network monitoring and advanced methods in plant microbiome engineering. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.

Authoritative and cutting-edge, Plant Microbiome Engineering aims to provide well-established protocols and procedures largely used by both academics and industrials.

This volume provides a comprehensive overview of Isolation of G+Ve Food Borne Pathogens, detection of their toxins by various approaches like Traditional methods, Spectrophotometric tool, Nucleic acid assay methods, Immunological assay methods and Biosensor approaches. Chapters detail rapid detection of notable pathogen such as Bacillus cereus by Molecular approach.  A special mention here about the Entrapment of Gram Positive Pathogens from food sample by Dielectrophoresis method by Lab designed Electronic chip. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.

Authoritative and cutting-edge, Gram Positive Bacterial Food Borne Pathogens aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins.  It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS.  A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book.

In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin.

Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research.