Alison J.M. Hill – Författare
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3 produkter
3 produkter
1 062 kr
Skickas inom 10-15 vardagar
DNA Sequencing Protocols, Second Edition, empowers researchers with the tools and knowledge required to become a sequencing expert. It covers a wide range of DNA sequencing technologies in common usage in molecular biology laboratories. It acts as a laboratory guide to DNA sequencing and it helps students and researchers to understand the instrumentation (ABI, ALF & LiCor) and chemistries involved. The reader is also given internet addresses for Genome sequencing sites and step by step guides to the use of sequence analysis software and databases i. e. for BLAST and NIX searches. The book also reviews some of the instrumentation available for automation of DNA sequencing processes which is in more widespread use in many research groups. Some of the protocols covered in detail include, M13 cloning and sequencing; Primer design; PCR product sequencing; Cycle sequencing; Solid phase sequencing; Shotgun sequencing; Fluorescent sequencing for 310, 373, 377, ALF, LiCor; Heterozygote mutation detection; Automation, and; Sequence databases and the internet.The book will also be useful to clinicians with research interests to update them on current DNA sequencing methods, applications and future prospects. DNA sequencing has become an essential tool for molecular diagnostics with the completion of many genome project.
1 062 kr
Skickas inom 10-15 vardagar
Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.
1 378 kr
Skickas inom 10-15 vardagar
Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.