DNA Sequencing Protocols

Häftad, Engelska, 2011

Del i serien Methods in Molecular Biology

1 378 kr

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Beskrivning

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Produktinformation

Utgivningsdatum:2011-12-12
Mått:152 x 229 x 15 mm
Vikt:376 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:256
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781617371509

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Recensioner i media

"DNA Sequencing Protocols covers the commonly used protocols in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers involved in any aspect of DNA sequencing." - E-STREAMS

Innehållsförteckning

Introduction to DNA Sequencing. The Universal Primers and the Shotgun DNA Sequencing Method. M13 Sequencing. Primer Design and Primer-Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene. Shotgun DNA Sequencing. Cycle Sequencing. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. Fluorescent Sequencing Protocols for the ALF. Fluorescent Sequencing for Heterozygote Mutation Detection. Sequence Databases and the Internet. DNA Sequencing by Capillary Array Electrophoresis. Index.