This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today''s leaders in the field ofprotein engineering. Chapters focus on modeling protein ligand bindingsites, accurate modeling of protein-ligand conformational sampling, scoring ofindividual docked solutions, structure-based design program such as ROSETTA,protein engineering, and additional methodological approaches. Examples of applicationsinclude the design of metal-binding proteins and light-induced ligand bindingproteins, the creation of binding proteins that also display catalyticactivity, and the binding of larger peptide, protein, DNA and RNAligands. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips ontroubleshooting and avoidingknown pitfalls.

This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.

This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today''s leaders in the field ofprotein engineering. Chapters focus on modeling protein ligand bindingsites, accurate modeling of protein-ligand conformational sampling, scoring ofindividual docked solutions, structure-based design program such as ROSETTA,protein engineering, and additional methodological approaches. Examples of applicationsinclude the design of metal-binding proteins and light-induced ligand bindingproteins, the creation of binding proteins that also display catalyticactivity, and the binding of larger peptide, protein, DNA and RNAligands. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips ontroubleshooting and avoidingknown pitfalls.

This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.