Buddhi Prakash Jain – författare

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

Understanding Cancer: From Basics to Therapeutics presents both basic concepts and research prospects in the field of cancer biology. This book summarizes the fundamental aspects of cancer and presents a detailed description of molecular aspects as well as treatment and therapeutics for patients.

The book is divided into three parts: the first part deals with the basics of cancer, including etiology and medical diagnosis; the second part explores the molecular mechanisms associated with cancer, focusing on cell cycle and apoptosis, cell metabolism, gene regulation, epigenetics, and stem cells; and the third part is dedicated to therapeutics, discussing chemo and radiotherapies, gene, hormone, herbal, and immunotherapies.

It is a valuable resource for cancer researchers, oncologists, graduate students, and biomedical researchers who need to understand the fundamental topics related to cancer to apply to their research work or clinical setting.

Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.

Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.