Shyamal K Goswami – författare

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting Features clear, step-by-step protocols and instructions with color diagrams and images

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.- Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting- Features clear, step-by-step protocols and instructions with color diagrams and images

Post-transcriptional Gene Regulation in Human Disease, a new volume in the Translational Epigenetics book series, offers a thorough overview and discussion of post-transcriptional genetic control mechanisms and their roles across various pathologies and human developmental outcomes, along with regulatory mechanisms targeted for therapeutic approaches. The book is broadly divided in two parts: early chapters describe the basics of post-transcriptional gene regulation, associated epigenetic mechanisms, the role of RNA binding proteins, the evolution of post-transcriptional gene regulation, and methods to study these mechanisms. The second half of the book includes deeper discussion of post-transcriptional gene regulation across specific diseases and therapeutics targets. Various post-transcriptional events, including alternative splicing and polyadenylation, mRNA stability, and miRNAs and their involvement in the disease progression, are examined in detail. Includes full-color imagery illustrating key concepts and post-transcriptional disease processes, as well as descriptions of methods for studying post-transcriptional gene regulation Presents fundamental knowledge, molecular and biochemical mechanisms, and recent findings in concise and easily understandable formats Features a summary and conclusion at the end of each chapter

Post-transcriptional Gene Regulation in Human Disease, a new volume in the Translational Epigenetics book series, offers a thorough overview and discussion of post-transcriptional genetic control mechanisms and their roles across various pathologies and human developmental outcomes, along with regulatory mechanisms targeted for therapeutic approaches. The book is broadly divided in two parts: early chapters describe the basics of post-transcriptional gene regulation, associated epigenetic mechanisms, the role of RNA binding proteins, the evolution of post-transcriptional gene regulation, and methods to study these mechanisms. The second half of the book includes deeper discussion of post-transcriptional gene regulation across specific diseases and therapeutics targets. Various post-transcriptional events, including alternative splicing and polyadenylation, mRNA stability, and miRNAs and their involvement in the disease progression, are examined in detail.- Includes full-color imagery illustrating key concepts and post-transcriptional disease processes, as well as descriptions of methods for studying post-transcriptional gene regulation- Presents fundamental knowledge, molecular and biochemical mechanisms, and recent findings in concise and easily understandable formats- Features a summary and conclusion at the end of each chapter

Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.

Includes full listings and discussions of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting across 100 protocolsFeatures clear, step-by-step instruction with color diagrams and images, followed by worked examples of putting lab techniques into actionEmpowers researchers and clinicians to reproduce research and clinical methods and ensure test accuracy

Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.- Includes full listings and discussions of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting across 100 protocols- Features clear, step-by-step instruction with color diagrams and images, followed by worked examples of putting lab techniques into action- Empowers researchers and clinicians to reproduce research and clinical methods and ensure test accuracy

Fundamentals of Redox Biology is a comprehensive guide to understanding the complex roles of cellular redox systems in biological processes and human health. Exploring the regulation, physiological functions, and pathological implications of reactive oxygen species (ROS) and free radicals, this book serves as an essential resource for students and professionals in biochemistry and related fields. It delves into the fundamentals of redox signaling, the impact of antioxidants on homeostasis, and the intricate balance between oxidative stress and disease development, including conditions like cancer and neurological disorders. This foundational text is invaluable for advancing knowledge in redox biology.In addition to providing a historical perspective on redox biology, the book examines how ROS influence epigenetic regulation and mitochondrial function, particularly in immune responses to infections. It also integrates discussions on the role of redox systems across multiple disciplines, making it relevant to cell biology, molecular biology, immunology, and beyond. With its detailed exploration of both theoretical and applied aspects, the book positions itself as a pivotal resource for understanding the mechanisms underlying redox balance and its broad applications in life sciences. - Examines the role of free radicals and reactive species (ROS) in human health and disease- Explores redox biology in the context of humans, plants, Zebrafish, and Drosophila- Considers tools and techniques for the detection and estimation of cellular ROS- Looks at the role of ROS in genetic and epigenetic regulations- Features learning objectives and end-of-chapter problems to aid understanding