Daniela Giordano - Böcker

This detailed volume provides an overview of recent advances in the application of genomic technologies in several domains of marine biology, raising awareness of various DNA- and RNA-based technologies.

This detailed volume provides an overview of recent advances in the application of genomic technologies in several domains of marine biology, raising awareness of various DNA- and RNA-based technologies. Genomic methods are essential in identifying previously undetected taxonomic (e.g. DNA barcoding), genetic (e.g. sequencing), and functional (e.g. gene expression, analysis of metabolites) diversity, as shown in the chapters of this book, with sections focusing on next generation sequencing (NGS) technologies, bioinformatics in marine genomics research, marine biotechnology, as well as a variety of methods successfully applied in fish. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Marine Genomics: Methods and Protocols highlights the utility of numerous lab protocols and their potential to provide deeper insight into physiological and ecological mechanisms in marine life.

This bookgives a start-to-finish overview of the whole Fish4Knowledge project, in 18short chapters, each describing one aspect of the project. The Fish4Knowledgeproject explored the possibilities of big video data, in this case fromundersea video. Recording and analyzing 90 thousand hours of video from tencamera locations, the project gives a 3 year view of fish abundance in severaltropical coral reefs off the coast of Taiwan. The research system built aremote recording network, over 100 Tb of storage, supercomputer processing,video target detection and tracking, fish species recognition and analysis, alarge SQL database to record the results and an efficient retrieval mechanism.Novel user interface mechanisms were developed to provide easy access formarine ecologists, who wanted to explore the dataset. The book is a usefulresource for system builders, as it gives an overview of the many new methodsthat were created to build the Fish4Knowledge system in a manner that alsoallows readers to see how all the components fit together.