Marine Genomics

Methods and Protocols

AvCinzia Verde,Daniela Giordano

Inbunden, Engelska, 2022

Del i serien Methods in Molecular Biology

2 325 kr

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Beskrivning

This detailed volume provides an overview of recent advances in the application of genomic technologies in several domains of marine biology, raising awareness of various DNA- and RNA-based technologies.

Produktinformation

Utgivningsdatum:2022-06-22
Mått:178 x 254 x 30 mm
Vikt:1 027 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:426
Förlag:Springer-Verlag New York Inc.
ISBN:9781071623121

Utforska kategorier

Innehållsförteckning

Mitochondrial Genome of Non-Model Marine Metazoans by Next-Generation Sequencing (NGS).- Genome-Wide DNA Methylation Protocol for Epigenetics Studies.- Transcriptome Mining to Identify Genes of Interest: From Local Databases to Phylogenetic Inference.- Detecting Structural Variants and Associated Gene Presence-Absence Variation Phenomena in the Genomes of Marine Organisms.- From Sequences to Enzymes: Comparative Genomics to Study Evolutionary Conserved Protein Functions in Marine Microbes.- VenomFlow: An Automated Bioinformatic Pipeline for Identification of Disulfide-Rich Peptides from Venom Arsenals.- Population Genomics Analysis with RAD, Reprised: Stacks 2.- A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses.- Barcoding of Antarctic Marine Invertebrates: From Field Sampling to Lab Procedures.- DNA Barcoding Procedures for Taxonomical and Phylogenetic Studies in Marine Animals:Porifera as Case Study.- Environmental DNA from Marine Waters and Substrates: Protocols for Sampling and eDNA Extraction.- Metataxonomic Analysis of Bacterial Diversity Associated with Marine Organisms.- From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes.- Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria.- Isolation of UV-Resistant Marine Bacteria by UV-C Assays.- Fractionation Protocol of Marine Metabolites.- Detection and Quantification of Small Non-Coding RNAs in Marine Diatoms.- Optimized Proteolistic Protocol for the Delivery of the Cas9 Protein in Phaeodactylum tricornutum.- Production of a Chimeric Mouse-Fish Monoclonal Antibody by the CRISPR/Cas9 Technology.- Identification, Characterization, and Expression Analysis of Immunoglobulin Genes from Antarctic Fish by PCR Methods.- Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH).- Functional Genomics of Fish Erythrocytes.- Stain-Free Approach for Western Blot Analysis of Zebrafish Embryos.- Proteomics of Fish White Muscle and Western Blotting to Detect Putative Allergens.- In Vitro Assays for the Bifunctional Acylpeptide Hydrolase (APEH) Enzyme from Antarctic Fish.