David M. Prescott – författare

Cell Biology, A Comprehensive Treatise, Volume 3: Gene Expression: The Production of RNA''s mainly discusses the molecular and cytological bases of gene expression. The coverage begins with the concepts of organization of DNA and gene sequences in chromosomes, as an introduction to a more detailed coverage of gene expression. The book opens with a general discussion on the organization of DNA sequences in chromosomes. This chapter includes different methods of analyzing DNA sequences. As the book progresses, it looks upon the details on gene reiteration and amplification up to the transcription of prokaryotes and eukaryotes. It includes the ways of regulating transcription. The following chapters deal mostly with the structure and activity of genes up to the different virus strains in both RNA and DNA. The cytoplasmic and environmental impact on gene expression is also discussed. Chapter 8 generally tackles the DNA conformation and template function. The succeeding chapters focus on the transfer and ribosomal RNA as a result of maturation events; the processing of hnRNA and its relation to mRNA; and recombinant DNA procedures. The book closes with the directory of the different classes of cellular RNAs. This book will be helpful to many graduate students, teachers, scientists, and researchers in need of information regarding cell biology.

Cell Biology, A Comprehensive Treatise, Volume 4: Gene Expression: Translation and the Behavior of Proteins discusses the translation of genetic information into proteins. It also explains the activities and behaviors of proteins, which is the final stage of gene expression. This book opens with a discussion of the components and mechanism of the translational machinery – their structures, biogenesis, and cellular levels. It then explains the protein synthesis, wherein each chapter focuses on one aspect of the detailed process. Other topics covered in this book are the structure and function of cytoplasmic ribonucleoprotein complexes and the proteolytic cleavage in the posttranslational processing of proteins. This text also covers the regulation of enzyme activity and the movement of material between nucleus and cytoplasm. The last three chapters are more in-depth discussions on protein synthesis, intracellular transport, regulation, and secretion. As with the other volumes of this book series, readers will definitely benefit from the vast information provided in this book regarding protein, its activities, behaviors, and synthesis.

Cell Biology, A Comprehensive Treatise, Volume 2: The Structure and Replication of Genetic Material is mainly about the structure and replication of genetic material in both the nucleus and cytoplasmic organelles. This volume is part of the first four volumes that establish a firm foundation regarding issues of cell structure and function. These issues include cell reproduction, differentiation, and cell-to-cell interactions. This book is divided into nine chapters. Each chapter deals extensively with chromosomes – its physical, genetic, and chemical structures. In addition, this book explains the replication of chromosomes in terms of the cell cycle, as well as their coding capacity. It also discusses the functional organization (structure and levels) of the chromosomes. The concluding chapters present the DNA replication molecular principles and enzymatic machinery. Furthermore, this book explains DNA repair and its relationship to various biological endpoints. The authors of this book reasonably explain and emphasize already established facts and concepts in terms that are relatively easy to understand. Undergraduate and graduate students, teachers, researchers, scientists, and others interested or in need of information regarding cell biology will find this book of great use.

Reproduction of Eukaryotic Cells organizes in a single source the principal facts and observations on the cell life cycle and reproduction of eukaryotic cells. The aim is to increase the overall understanding of how these cells reproduce themselves and how this reproduction is regulated. The book begins with a discussion of the sections of the cell cycle and regulation of cell reproduction. Separate chapters on cell growth, cell synchrony, the G1 period, S period, and G2 period follow. Subsequent chapters are devoted to activities during cell division; cell cycle changes in surface morphology; the role of cyclic AMP (cAMP) and cyclic GMP (cGMP) in regulation of cell reproduction; and changes in nuclear proteins, RNA synthesis, and enzyme activities during the cell cycle. The final chapter covers the genetic analysis of the cell cycle.

Advances in Cell Biology has been initiated as a continuing, multi-volume series to report on the progress of a wide spectrum of problems of cell structure and cell function. Jn arranging these volumes individual contributors are asked not only to review the major new information, but especially to present the state of a given problem or area by discussing the current central issues, speculations, concepts, hypotheses, and technical problems. We intend, in addition, that these volumes will not be concerned with comprehensive reviews of the recent literature but will consist rather of presentations of an interpretive and integrative nature, based on selection of major research advances. It is our aim that these volumes should provide the means whereby cell biologists may keep themselves reasonably well informed about the current progress in research areas in cell biology in which they are not immediately or directly involved themselves. The articles, nevertheless, are expected to bring into focus the experimental objectives of the specialists in a given research area. D. M.P. L. G. E.M. vii Contents Contributors v Preface vii 1 1. The Regulation of DNA Synthesis in Eukaryotes James Douglas Watson 2. D·RNA Containing Ribonucleoprotein Particles and Messenger RNA Transport 47 G. P. Georgiev and 0. P. Samarina Recent Developments in the Synchronization of 3. Tetrahymena Cell Cycle 111 Eric Zeuthen 153 4. Repetitious DNA Christopher Bostock 5. Mitosis 225 R. Bruce Nicklas Specific Enzyme Production in Eukaryotic Cells 299 6.

Advances in Cell Biology has been initiated as a continuing, multi-volume series to report on the progress of a wide spectrum of problems of cell structure and cell function. Jn arranging these volumes individual contributors are asked not only to review the major new information, but especially to present the state of a given problem or area by discussing the current central issues, speculations, concepts, hypotheses, and technical problems. We intend, in addition, that these volumes will not be concerned with comprehensive reviews of the recent literature but will consist rather of presentations of an interpretive and integrative nature, based on selection of major research advances. It is our aim that these volumes should provide the means whereby cell biologists may keep themselves reasonably well informed about the current progress in research areas in cell biology in which they are not immediately or directly involved themselves. The articles, nevertheless, are expected to bring into focus the experimental objectives of the specialists in a given research area. D. M.P. L. G. E.M. vii Contents Contributors v Preface vii 1 1. The Regulation of DNA Synthesis in Eukaryotes James Douglas Watson 2. D·RNA Containing Ribonucleoprotein Particles and Messenger RNA Transport 47 G. P. Georgiev and 0. P. Samarina Recent Developments in the Synchronization of 3. Tetrahymena Cell Cycle 111 Eric Zeuthen 153 4. Repetitious DNA Christopher Bostock 5. Mitosis 225 R. Bruce Nicklas Specific Enzyme Production in Eukaryotic Cells 299 6.

Advances in Cell Biology has been initiated as a continuing, multi-volume series to report on the progress of a wide spectrum of problems of cell structure and cell func­ tion. In arranging these volumes individual contributors are asked not only to review the major new information, but especially to present the state of a given problem or area by discussing the current central issues, speculations, concepts, hypotheses, and technical problems. We intend, in addition, that these volumes will not be concerned with comprehensive reviews of the recent literature but will consist rather of presenta­ tions of an interpretative and integrative nature, based on selection of major research advances. It is our aim that these volumes should provide the means whereby cell biologists may keep themselves reasonably well informed about the current progress in research areas in cell biology in which they are not immediately or directly involved themselves. The articles, nevertheless, are expected to bring into focus the experimental objectives of the specialists in a given research area. D.M.P. L. G. E. M. vii Contents v Contributors Preface vii Bacterial Chromosome Replication 3 I. Peter L. Kuempel 57 2. Structure and Replication of Eukaryotic Chromosomes David M. Prescott Ultrastructure and Interaction of the Kinetochore and Centriole 3.

Methods in Cell Physiology

Methods in Cell Physiology

Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included. The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.

Natural Computing is concerned with computation that is taking place in Nature. The investigation of computations in living cells is one of the central and fastest growing areas of research in this field. Gene assembly in ciliates (unicellular organisms) is a splendid example of such computations, and it is fascinating from both the biological and the computational viewpoints. As a matter of fact, both biology and the science of computation have benefited from the interdisciplinary research on the computational nature of gene assembly – this work has helped to clarify important biological aspects of gene assembly, yielded novel insights into the nature of computation, and broadened our understanding of what computation is about. This monograph gives an accessible account of both the biology and the formal analysis of the gene assembly process. It can be used as a textbook for either graduate courses or seminars.

Natural Computing is concerned with computation that is taking place in Nature. The investigation of computations in living cells is one of the central and fastest growing areas of research in this field. Gene assembly in ciliates (unicellular organisms) is a splendid example of such computations, and it is fascinating from both the biological and the computational viewpoints. As a matter of fact, both biology and the science of computation have benefited from the interdisciplinary research on the computational nature of gene assembly – this work has helped to clarify important biological aspects of gene assembly, yielded novel insights into the nature of computation, and broadened our understanding of what computation is about. This monograph gives an accessible account of both the biology and the formal analysis of the gene assembly process. It can be used as a textbook for either graduate courses or seminars.