David Stirling – författare

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

This book constitutes the refereed proceedings of the 15th Australasian Conference on Data Mining, AusDM 2017, held in Melbourne, VIC, Australia, in August 2017.The 17 revised full papers presented together with 11 research track papers and 6 application track papers were carefully reviewed and selected from 31 submissions. The papers are organized in topical sections on clustering and classification; big data; time series; outlier detection and applications; social media and applications.

This book constitutes the refereed proceedings of the 16th Australasian Conference on Data Mining, AusDM 2018, held in Bathurst, NSW, Australia, in November 2018.The 27 revised full papers presented together with 3 short papers were carefully reviewed and selected from 80 submissions. The papers are organized in topical sections on classification task; transport, environment, and energy; applied data mining; privacy and clustering; statistics in data science; health, software and smartphone; image data mining; industry showcase.