G Fischer - Böcker

The book is on methods of reconstruction of past climate, climate changes, oceanography of the South Atlantic (including the Southern Ocean) and the cycling of biochemical components in the ocean. Information about past ocean conditions is provided by so-called paleoceanographic proxies (parameters with approximate unobservable environmental variables, e.g. surface water temperature). Use of proxies and their development has been a major theme of the Collaborative Research Project at the University of Bremen for almost 10 years. In this volume we summarize our results in relation to these proxy studies. Each chapter gives an overview of a specific proxy, provides particular findings obtained in the South Atlantic and ends with an outlook on future perspectives with respect to the proxy described. Therefore it is suitable for lecturers, graduate students and scientists working in the field of climate reconstruction from ocean sediments.

Until some years ago serum or crude tissue extracts were used pre­ dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup­ plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer­ ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re­ gard to survival, proliferation and differentiation of cells in cul­ ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti­ vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.