Govind Pratap Rao – författare

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

The book is a compilation of research work carried out on plant viruses during past 100 years in India. Plant viruses are important constraints in Indian agriculture. Tropical and sub-tropical environments and intensive crop cultivation practices ideally favours perpetuation of numerous plant viruses and their vectors in India, which often cause wide spread crop losses. Of all the plant pathogens, studies of plant viruses have received a special attention as they are difficult to manage. A large body of literature has been published on the plant virus research from India during past 100 years; however the information is so far not available in one place. This book provides comprehensive information on the biology, molecular biology, epidemics, crop losses, diagnosis and management of viruses and viroids occurring in India. Description of properties of the viruses are provided in the chapters comprising of  different genera such as Allexivirus, Begomovirus, Babuvirus, Badnavirus,Carlavirus, Carmovirus, Cucumovirus, Closterovirus, Ilavirus, Mandrivirus, Potyvirus, Tospovirus, Tungrovirus and Sobemovirus. Virus-vector research related to aphid, thrips and whitefly is discussed. The work on the management aspects of plant viral diseases has been described with reference to the conventional, antiviral and transgenic approaches. Further, the quarantine mechanism developed in India for the exclusion of viruses and vectors has also been included. The book also provides useful information about the capacity building on the research and education on Plant Virology in India. Overall, the book covers a wide range of accounts of research findings and innovations in Plant Virology in India during past 100 years. The book will be a resourceful reference to the students, scientists, agricultural professionals and policy makers.

Phytoplasma-associated diseases are a major limiting factor to quality and productivity of many ornamentals, horticultural and other economically important agriculture crops worldwide. Annual losses due to phytoplasma diseases in many crops vary, but under the pathogen favorable conditions they always lead to disastrous consequences to farming community. As there is no effective cure for phytoplasma diseases, the management options emphasize on their exclusion, minimizing their spread by insect vectors and propagation materials and on development of host plant resistance. The phytoplasma associated plant diseases have a history of more than 50 years. Phytoplasmas have undoubtedly infected plants and cause diseases for centuries before they are described and proven to be the causal agents. But important progress related to identification of phytoplasmas only began after 1980’s. Phytoplasmas have emerged as the most serious constraints in the production of several crops all around theworld during last four decades. Phytoplasmas constitute a major limiting factor to quality and productivity of cereals, horticultural, ornamentals and many other economically important crops all over the world. Annual losses due to phytoplasma diseases may vary, but under the pathogen favorable condition, phytoplasma disease may lead to disastrous consequences for farming and industry community. The scientific literature concerning phytoplasma occurrence, characterization, diagnosis, detection, and management is growing at a fast pace.            

Significant advancement in the last decades on diagnostic, biological and molecular properties, epidemiology, host-pathogen-insect interactions as well as management of phytoplasmas has been made. Till date, no authentic compilation is available to know the progress of phytoplasmas characterization major crops all over the world. The planned book will compile all the updated informationavailable information on phytoplasmas by distinguished experts in the form of edited book entitled “Characterization and epidemiology of phytoplasma associated diseases”. The book covers recent and update information on emerging and re-emerging phytoplasma diseases affecting important crops in tropics and subtropics. It provides comprehensive information on disease distribution, occurrence, and identification of the phytoplasmas including the recent approaches for diagnostics, transmission, and information about losses and geographical distribution along with and management aspects. This volume contains 11-12 chapters contributed by the experienced and recognized experts working on different group of phytoplasmas affecting major crops all over the world. The information on various topics is at advanced as well as comprehensive level and provides the period wise developments of phytoplasma research. The book covers major chapters on an up to date progress of phytoplasma research, and then phytoplasma diseases associated with vegetable, pulse, oils crops, cereals, sugar crops, fruit crops, ornamentals, medicinal plants, palms species, forest tress and weeds. We have covered historical background, geographical distribution, identification and characterization, genetic diversity, host pathogen interaction and management aspects of important phytoplasma diseases infecting our major agricultural crops.   

The information on various topics is advanced as well as comprehensive, and provides thought provoking ideas for planning novel research ideas for future. This book will be useful to everyone interested in mollicutes, phytoplasma, spiroplasmas, plant pathology, disease control and plant biology and serve as an exhaustive and up-to-date compendium of references on various aspects of different groups of phytoplasmas affecting important crops worldwide.

Phytoplasma III is the last of three books in the series covering all the aspects of phytoplasma-associated diseases. Phytoplasmas are a major limiting factor in the quality and productivity of many ornamental, horticultural and economically important agriculture crops worldwide, and losses due to phytoplasma diseases have disastrous consequences for farming communities. As there is no effective cure for these diseases, management strategies focus-on exclusion, minimizing their spread by insect vectors and propagation materials, and developing host plant resistance.