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5 produkter
5 produkter
Del 11 - Frontiers in Molecular Biology
Molecular Immunology
Häftad, Engelska, 1996
1 620 kr
Skickas inom 3-6 vardagar
Like other volumes in the Frontiers in Molecular Biology series, this book presents an up-to-date picture of current research topics in a key area. The contributors to this book have been carefully chosen to reflect the main thrusts of research effort across a broad range within the rapidly developing field of immunology. Subjects covered include immunoglobulin gene rearrangement, the MHC antigens, B-cell activation, and the complement system.
Del 148 - Practical Approach Series
DNA Cloning 1: A Practical Approach
Core Techniques
Häftad, Engelska, 1995
1 543 kr
Skickas inom 3-6 vardagar
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
Del 149 - Practical Approach Series
DNA Cloning 2: A Practical Approach
Expression Systems
Häftad, Engelska, 1995
1 543 kr
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Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
Del 202 - Practical Approach Series
Protein Expression
A Practical Approach
Häftad, Engelska, 1999
694 kr
Skickas inom 7-10 vardagar
Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
Del 197 - Practical Approach Series
Gel Electrophoresis of Proteins
A Practical Approach
Häftad, Engelska, 1998
1 543 kr
Skickas inom 3-6 vardagar
This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.