DNA Cloning 2: A Practical Approach

Expression Systems

AvHames Glover,D. M. Glover

Häftad, Engelska, 1995

Del 149 i serien Practical Approach Series

1 485 kr

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Beskrivning

Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.

Produktinformation

Utgivningsdatum:1995-05-11
Mått:157 x 235 x 15 mm
Vikt:465 g
Format:Häftad
Språk:Engelska
Serie:Practical Approach Series
Antal sidor:274
Upplaga:2
Förlag:IRL Press
ISBN:9780199634781

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a worthwhile bench top companion

Innehållsförteckning

1. Screening expression libraries with antibody and protein probes ; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies ; 3. Purification of over-produced proteins from E.coli cells ; 4. Production of monoclonal antibodies against proteins expressed in E.coli ; 5. Expression of cloned genes in yeast ; 6. Intraction trap cloning with yeast ; 7. The baculovirus expression system