Joachim W. Engels – författare

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins- Measuring enzymatic activity- Microcalorimetry- Immunoassays, affinity chromatography and other immunological methods- Cross-linking, cleavage, and chemical modification of proteins- Light microscopy, electron microscopy and atomic force microscopy- Chromatographic and electrophoretic techniques- Protein sequence and composition analysis- Mass spectrometry methods- Measuring protein-protein interactions- Biosensors- NMR and EPR of biomolecules- Electron microscopy and X-ray structure analysis- Carbohydrate and lipid analysis- Analysis of posttranslational modifications- Isolation and determination of nucleic acids- DNA hybridization techniques- Polymerase chain reaction techniques- Protein sequence and composition analysis- DNA sequence and epigenetic modification analysis- Analysis of protein-nucleic acid interactions- Analysis of sequence data- Proteomics, metabolomics, peptidomics and toponomics- Chemical biology

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird''s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird''s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Das bewährte Standardwerk beschreibt und erläutert alle analytischen Methoden, die heute in der Biochemie und Molekularbiologie eingesetzt werden, von der Probenvorbereitung bis zu modernsten Analysetechniken, von der Nucleotidanalytik bis zum Next Generation Sequencing, von der Analyse spezieller Stoffgruppen bis zur Systembiologie. Es begleitet Studierende der biologischen Disziplinen durch das gesamte Fächerspektrum und gibt ihnen das methodische Rüstzeug, den Erkenntnisgewinn in den Lebenswissenschaften zu durchdringen. Auch dem aktiv forschenden Wissenschaftler erläutert es die komplexen aktuellen Entwicklungen der bioanalytischen Methoden. In der Neuauflage sind fast alle Kapitel komplett überarbeitet und auf den neuesten Stand der Technik gebracht worden. Auch gänzlich neue Themen sind in der Neuauflage aufgenommen worden, wie beispielsweise die modernsten massenspektrometrischen Methoden für die OMICs-Techniken, CRISPR/Cas oder die Organ-on-a-Chip Technologie. Es berücksichtigt zahlreiche methodische Weiterentwicklungen und greift auch hochaktuelle Trends in der Forschung auf. Großer Wert wurde auf eine kritische, praxisbezogene Darstellung der Methoden und auf eine Vernetzung der verschiedenen Kapitel untereinander gelegt. Didaktisch überarbeitete Vierfarbabbildungen illustrieren durchgängig die beschriebenen Techniken. Damit wird die Neuauflage dieses kompetenten und informationsreichen Lehr- und Handbuches wieder all jenen, die sich in der Vielfalt der biologisch-chemischen Labormethoden zurechtfinden müssen, als zuverlässiger Wegweiser dienen. Vom Einsteiger bis zum erfahrenen Experten wird jeder Leser wertvolle Informationen finden.Stimmen zum BuchDas vielgelobte Buch … Das Buch ist der Klassiker der Bioanalytik. Alles, was ein Masterstudent über dieses Gebiet wissen muss, ist hier gründlich erklärt. Isabelle Breloy Hochschule Bonn-Rhein-SiegKann vorbehaltlos für einen weiten Kreis interessierter Leser (vom Studenten bis zum Lernenden, vom Forschenden bis zum Industrieanwender) sehr empfohlen werden. Angewandte ChemieEin Lehrbuch, das ... im deutschsprachigen Raum seinesgleichen sucht. ErnährungsforschungIn seinem Umfang einzigartig, komplette Methodenübersicht, topaktuell. Prof. Dr. Peter Fischer, Technische Fachhochschule Berlin... liegt nun in der vierten, aktualisierten und erweiterten Neuauflage vor.