Julia Jones - Böcker

This laugh-out-loud debut romcom about trying to keep lust alive in long-term relationships is perfect for fans of Sophie Kinsella, Mhairi McFarlane and Lindsey Kelk.'Hilarious, heartfelt and unputdownable! Handles the complications of marriage, sex and friendships with wit and warmth. I loved it!'Laura Kay, author of The Split and Wild Things'I absolutely devoured this book. The characters feel so real that it felt more like catching up with old friends over brunch than reading. Theo can spice up my legumes any time he likes!'Holly McCulloch, author of Just FriendsLottie and Theo are definitely happily married: deeply in love, beautiful house, two adorable puppies… except that after seven years together, the only action they’re having in the bedroom is Netflix marathons. Why has every night become ‘not tonight’?Frustrated in more ways than one, Lottie drunkenly calls in to her favourite podcast (The Cliterati) to let off some steam. But her anonymous rant goes viral, with thousands of women replying with tales of their own long-term relationships going limp and floppy.Now everyone is trying to unmask the ‘horny wife’, the podcast agency make Lottie an offer which could change her life. The only problem is… she still hasn’t told Theo about her confession. How on earth can Lottie admit to her husband that they could be famous – for being sexless?The laugh-out-loud romcom about love, friendship, and trying to keep lust alive in long-term relationships, perfect for fans of Sophie Kinsella, Mhairi Macfarlane and Lindsey Kelk.

This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field.

This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field.

This sixth edition volume expands on the previous editions with a discussion of the latest methods used to detect RNA or DNA species in situ, including both short and long RNAs, and coding and non-coding RNAs. The chapters in this book are organized into five parts: RNA detection techniques; RNA and protein co-detection; 3D and wholemount ISH; spatial transcriptomics approaches; and image analysis and quantification. Some of the topics covered in this volume are detection of viral pathogens using RNAscope™; detection of therapeutic antisense oligonucleotides using miRNAscope assays; RNAscope HiPlex in tissue sections and wholemounts; spatial transcriptomics platforms with accompanying data processing; and various image analysis platforms for downstream quantitative assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, In Situ Hybridization Protocols, Sixth Edition brings together a collection of protocols, along with practical notes and recommendations, and offers researchers a comprehensive introduction to the current leading edge of in situ hybridization.

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The extraordinary stories of the fearless women who set their sights on the sea in an all-male world, paving the way for the female sailing superstars of today.Gender issues are stark at sea. Historically a ship’s crew is all male, and although vessels are referred to as ‘she’, women’s contributions to sailing have been largely ignored, if not actively opposed. Today’s sailing legends such as Tracy Edwards and Ellen MacArthur compete with men on equal terms, but they stand on the shoulders of many women before them, who had to challenge preconceptions, prejudice and even the law in their wish to sail.Spanning the 19th century to today, Julia Jones dives into the lives of an array of incredible and unconventional women: we meet single-handed sailors, wealthy explorers, long-suffering wives and penniless chancers with a thirst for adventure. Each had to face down barriers of official exclusion, family-imposed restriction, social disapproval and often a resulting lack of self-belief.In the 21st-century, British women wear Admiral’s uniforms and break global yachting records, yet their journey to professional sailing remains challenging, and the public face of sailing remains mostly male – evident in the lack of female Commodores in prestigious yacht clubs. Even as sailing becomes more accessible, the remarkable contributions within these stories are a precious source of inspiration to all women facing their own challenges today.

'An extraordinary account of heroism and sacrifice. An unexpected and important story, rivetingly told. Rip roaring stuff. Get this into the paws of the sea dog in your life.' - Griff Rhys Jones'A book that had to be written' - Let's Talk'People ashore don’t realise what a grim war we are waging at sea with the Germans. A cold-blooded war, in a way I think requiring the maximum of bravery from the men of both sides in the long run, as it is so ceaseless and intangible. You just don’t know whether the next moment will be your last.' Robert Hichens, RNVSR Several years ago, Julia Jones was searching through long-forgotten items stored at her house and discovered some suitcases of old written material, which turned out to be accounts by her father of his experiences in the RNVSR (Royal Naval Volunteer Supplementary Reserve). She realised that as a child she’d met some of the people mentioned, and although she was too young to truly know them, these youthful impressions spurred her on to rediscovery and understanding. In this absorbing book Julia tells the compelling stories of the yachtsmen. Some were famous (such as Sir Peter Scott), others were wealthy (such as August Courtauld, who returned his pay to help with the war effort) but the majority were just 'ordinary' professionals such as publishers, lawyers and advertising agents, who signed up because they loved sailing. Few could ever have dreamed that they would end up acting in areas that were so far beyond their normal lives, as they found themselves commanding destroyers and submarines, and undertaking covert missions of sabotage. Some undertook the dangerous daily drudgery of minesweeping; others tackled unexploded bombs, engaged the enemy in high-speed attacks or played key roles in Ian Fleming's famous intelligence commandos. This varied crew of men were given tasks vital to the war effort, requiring endurance, extraordinary bravery, resourcefulness and quick thinking. Some died in the process, but for the ones who survived, Julia asks how their experiences changed them. Could their love of sailing and the sea survive the harsh realities of war?

A central goal of transportation is the delivery of safe and efficient services with minimal environmental impact. In practice, though, human mobility has flourished while nature has suffered. Awarness of the environmental impacts of roads is increasing, yet information remains scarce for those interested in studying, understanding, or minimizing the ecological effects of roads and vehicles. Road Ecology addresses that shortcoming by elevating previously localized and fragmented knowledge into a broad and inclusive framework for understanding and developing solutions. The book brings together fourteen leading ecologists and transportation experts to articulate state-of-the-science road ecology principles and presents specific examples that demonstrate the application of those principles.