Ratan Kumar Choudhary – författare

An accessible guide that introduces students in all areas of life sciences to bioinformaticsBasic Applied Bioinformatics provides a practical guidance in bioinformatics and helps students to optimize parameters for data analysis and then to draw accurate conclusions from the results. In addition to parameter optimization, the text will also familiarize students with relevant terminology. Basic Applied Bioinformatics is written as an accessible guide for graduate students studying bioinformatics, biotechnology, and other related sub-disciplines of the life sciences. This accessible text outlines the basics of bioinformatics, including pertinent information such as downloading molecular sequences (nucleotide and protein) from databases; BLAST analyses; primer designing and its quality checking, multiple sequence alignment (global and local using freely available software); phylogenetic tree construction (using UPGMA, NJ, MP, ME, FM algorithm and MEGA7 suite), prediction of protein structures and genome annotation, RNASeq data analyses and identification of differentially expressed genes and similar advanced bioinformatics analyses. The authors Chandra Sekhar Mukhopadhyay, Ratan Kumar Choudhary, and Mir Asif Iquebal are noted experts in the field and have come together to provide an updated information on bioinformatics.Salient features of this book includes: Accessible and updated information on bioinformatics toolsA practical step-by-step approach to molecular-data analyses Information pertinent to study a variety of disciplines including biotechnology, zoology, bioinformatics and other related fieldsWorked examples, glossary terms, problems and solutionsBasic Applied Bioinformatics gives students studying bioinformatics, agricultural biotechnology, animal biotechnology, medical biotechnology, microbial biotechnology, and zoology an updated introduction to the growing field of bioinformatics.

Biological fluids such as blood, milk, fine needle aspirates, urine, and saliva from livestock play a critical role in veterinary medicine, animal husbandry, and agricultural research. These fluids serve as non-invasive or minimally invasive sources of valuable diagnostic information, enabling the monitoring of animal health, disease detection, and the assessment of physiological and metabolic states. Blood, the most widely studied fluid, provides insights into an animal overall health, immune status, and the presence of infections or metabolic disorders. Milk, particularly in dairy cattle, is crucial for assessing mammary gland health and detecting conditions like mastitis. Detection of milk somatic cells on a routine basis and keeping them low is desirable for milk and milk product quality. Fine needle aspirates (FNA) allow for the examination of specific tissues, such as lymph nodes and cancer masses, offering detailed information on cellular composition and the presence of pathological changes in cell morphology and cellular composition. Urine and saliva are increasingly recognized for their diagnostic potential. Urine analysis can reveal metabolic and kidney function and provide early indicators of systemic diseases. Saliva, being easy to collect, is a growing focus for non-invasive monitoring, hormonal changes, cancer, diseases and stress.

Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.

This book explores the potential applications of animal stem cells in veterinary medicine. In turn, the book discusses the challenges of using stem cells in regenerative medicine and emphasizes the importance of understanding the action of stem cells and preclinical evidence for ensuring safety and therapeutic efficacy.

This book explores the potential applications of animal stem cells in veterinary medicine. In turn, the book discusses the challenges of using stem cells in regenerative medicine and emphasizes the importance of understanding the action of stem cells and preclinical evidence for ensuring safety and therapeutic efficacy.

This book comprehensively discusses the applications of molecular genetics, functional and structural genomics, and proteomics vis-a-vis bioinformatics, artificial intelligence, and robotics in livestock healthfulness and productivity.