Ratan Kumar Choudhary College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaManu M College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaYashpal Singh Malik: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India.

• 1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities2 Basic Equipment of Animal Cell Culture Laboratories3 Washing, Packing, and Sterilization of Glass and Plastic Wares4 Preparation of Cell Culture Media and Reagents5 Isolation of Primary Culture of Chicken Embryo Fibroblast6 Isolation of Primary Culture of Canine Keratinocytes7 Isolation of Primary Culture of Canine Fibroblast8 Culture and Sub-culturing of Continuous Cell Lines9 Trypan Blue Dye Exclusion Cell Viability Assay10 Isolation and Culture Techniques for Lymphocytes11 Cryopreservation Methods of Primary Cultures and Cell Lines12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells13 Virus Isolation in Cell Culture System14 One-Step Growth Curve of a Virus15 Production of Virus in Cell Culture16 Quantitative Analysis of Virus Titers through In-Vitro Assays17 Hemadsorption (HA) Assay for Virus Titration18 Plaque Assay for Virus Detection and Quantification19 Virus Neutralization Test20 Techniques for Establishing and Maintaining Cell Suspension Cultures21 Development of Cell lines Through Virus-Mediated Techniques22 Cell Viability and Cell Proliferation Assay

Ratan Kumar Choudhary College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaManu M College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaYashpal Singh Malik: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India.

• 1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities2 Basic Equipment of Animal Cell Culture Laboratories3 Washing, Packing, and Sterilization of Glass and Plastic Wares4 Preparation of Cell Culture Media and Reagents5 Isolation of Primary Culture of Chicken Embryo Fibroblast6 Isolation of Primary Culture of Canine Keratinocytes7 Isolation of Primary Culture of Canine Fibroblast8 Culture and Sub-culturing of Continuous Cell Lines9 Trypan Blue Dye Exclusion Cell Viability Assay10 Isolation and Culture Techniques for Lymphocytes11 Cryopreservation Methods of Primary Cultures and Cell Lines12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells13 Virus Isolation in Cell Culture System14 One-Step Growth Curve of a Virus15 Production of Virus in Cell Culture16 Quantitative Analysis of Virus Titers through In-Vitro Assays17 Hemadsorption (HA) Assay for Virus Titration18 Plaque Assay for Virus Detection and Quantification19 Virus Neutralization Test20 Techniques for Establishing and Maintaining Cell Suspension Cultures21 Development of Cell lines Through Virus-Mediated Techniques22 Cell Viability and Cell Proliferation Assay

Ratan Kumar Choudhary College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaManu M College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, IndiaYashpal Singh Malik: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India.

• 1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities2 Basic Equipment of Animal Cell Culture Laboratories3 Washing, Packing, and Sterilization of Glass and Plastic Wares4 Preparation of Cell Culture Media and Reagents5 Isolation of Primary Culture of Chicken Embryo Fibroblast6 Isolation of Primary Culture of Canine Keratinocytes7 Isolation of Primary Culture of Canine Fibroblast8 Culture and Sub-culturing of Continuous Cell Lines9 Trypan Blue Dye Exclusion Cell Viability Assay10 Isolation and Culture Techniques for Lymphocytes11 Cryopreservation Methods of Primary Cultures and Cell Lines12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells13 Virus Isolation in Cell Culture System14 One-Step Growth Curve of a Virus15 Production of Virus in Cell Culture16 Quantitative Analysis of Virus Titers through In-Vitro Assays17 Hemadsorption (HA) Assay for Virus Titration18 Plaque Assay for Virus Detection and Quantification19 Virus Neutralization Test20 Techniques for Establishing and Maintaining Cell Suspension Cultures21 Development of Cell lines Through Virus-Mediated Techniques22 Cell Viability and Cell Proliferation Assay