Protocol Series – serie

The book Handbook on Basic Laboratory Skills is designed to serve two primary goals: (i) for teaching basic concepts of laboratory techniques and (ii) following good laboratory practices while working in laboratory or doing research. The book presents the importance of laboratory skills/practices and biosafety issues to students and researchers. The chapters included in this book cover information on handling of laboratory chemicals, equipment, preparation of buffers, media, basics of seed testing, biosafety, etc. which constitute curriculum of the course Basic Concepts in Laboratory Techniques (PGS504, 0L 1P) for Post-Graduate students of the Indian National Agricultural Research System (NARS) including Deemed Universities, State Agricultural Universities (SAUs), and Central Agricultural Universities (CAUs).

This handbook on food packaging experiments is an essential guide for students and professionals in food technology and packaging science. It offers a practical approach to understanding critical packaging concepts, from material selection and performance testing to shelf-life evaluation and safety measures. Designed to bridge theory with practice, this book provides step-by-step experimental procedures that enhance knowledge and skills in modern food packaging.

This book serves as a hands-on guide to foundational laboratory techniques in veterinary parasitology and molecular biology, specifically tailored for students, interns, and early-career researchers entering lab-based veterinary diagnostics. Divided into three focused chapters, the book begins with essential laboratory practices, including correct lab conduct, familiarization with basic lab equipment, and the preparation of core reagents and solutions necessary for routine experiments.The second chapter introduces key techniques in veterinary parasitology, such as proper sample collection and preservation, blood and fecal parasite detection, and the basics of serum biochemistry and immunodiagnostic assays. The third chapter provides a sequential understanding of molecular biology procedures relevant to parasitology researchranging from DNA extraction and primer design to PCR amplification and sequence analysis.In addition to theoretical instruction, the manual includes a practical appendix for equipment care, troubleshooting, and routine maintenanceensuring efficiency and accuracy in day-to-day laboratory operations. This concise and structured resource supports skill development and diagnostic competence in modern parasitological and molecular veterinary laboratories.

Experimental and Evidence based Protocols in Pharmacology provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures. Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application, DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen. This book imbibes all the basic and advanced research protocols along with their translational approaches in experiments involved in pharmacology and also provides the insight of example based drug assays to help in bioprospecting of naive molecules.Further, this book also provide a reference book to the researchers involved in toxicological testing using OECD testing guideline to calculate the lethal dose of any compound in a better manner. This MS also suffice the different methods to calculate the median lethal dose (LD50). The important reproductive toxicity assays to ascertain the toxic potential of any xenobiotics is also incorporated in this MS. Moreover, sophisticated technique like Flow cytometry and other experimental models viz. Direct and Indirect LPS induced acute lung injury model has also well written to provide an insight of the experimentation involving laboratory animals.

The book Handbook on Basic Laboratory Skills is designed to serve two primary goals: (i) for teaching basic concepts of laboratory techniques and (ii) following good laboratory practices while working in laboratory or doing research. The book presents the importance of laboratory skills/practices and biosafety issues to students and researchers. The chapters included in this book cover information on handling of laboratory chemicals, equipment, preparation of buffers, media, basics of seed testing, biosafety, etc. which constitute curriculum of the course Basic Concepts in Laboratory Techniques (PGS504, 0L 1P) for Post-Graduate students of the Indian National Agricultural Research System (NARS) including Deemed Universities, State Agricultural Universities (SAUs), and Central Agricultural Universities (CAUs).

Biological fluids such as blood, milk, fine needle aspirates, urine, and saliva from livestock play a critical role in veterinary medicine, animal husbandry, and agricultural research. These fluids serve as non-invasive or minimally invasive sources of valuable diagnostic information, enabling the monitoring of animal health, disease detection, and the assessment of physiological and metabolic states. Blood, the most widely studied fluid, provides insights into an animal overall health, immune status, and the presence of infections or metabolic disorders. Milk, particularly in dairy cattle, is crucial for assessing mammary gland health and detecting conditions like mastitis. Detection of milk somatic cells on a routine basis and keeping them low is desirable for milk and milk product quality. Fine needle aspirates (FNA) allow for the examination of specific tissues, such as lymph nodes and cancer masses, offering detailed information on cellular composition and the presence of pathological changes in cell morphology and cellular composition. Urine and saliva are increasingly recognized for their diagnostic potential. Urine analysis can reveal metabolic and kidney function and provide early indicators of systemic diseases. Saliva, being easy to collect, is a growing focus for non-invasive monitoring, hormonal changes, cancer, diseases and stress.

The book explores various categories of diseases affecting living organisms, including bacterial, viral, parasitic, and fungal diseases. Each category delves into the unique characteristics, causes, and impacts of these diseases, providing a comprehensive understanding of their significance and the challenges they pose to health and well-being.