Robert Aitken - Böcker

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.

A uniquely playful and incisive collection of Zen teaching stories from a beloved American master.A modern classic, now in a new expanded edition. In the tradition of the great koan collections and the records of ancient masters, Robert Aitken distills a lifetime of teaching down to its essence. Intriguing and deceptively simple, Zen Master Raven is a brilliant encapsulation of Zen in over a hundred koan-like encounters alongside many charming illustrations. Featuring curious beginners like Mallard and Mole and profound teachers like Brown Bear, Moose Roshi, and Zen Master Raven himself, this classic of contemporary Zen and will inspire seekers for generations to come.

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.

Zenbuddhismens väg är en ingående introduktion och handbok för nybörjaren, men även inspirerande för den erfarne utövaren. Steg för steg vägleder här den erfarne amerikanske zenmästaren Robert Aitken läsaren hur man utövar zazen, zenbuddhistisk meditation. Han beskriver olika sittställningar, hur man andas, frågor och problem man kan möta på vägen och studiet av koaner, dialoger och klassiska fall. Sedan Aitkens Taking the Path of Zen kom ut 1982, har den varit en av de främsta och mest lättillgängliga introduktionerna till zenbuddhism som skrivits.