Robert Aitken – författare

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

“In this, his final work, American senior Zen Roshi Robert Aitken lovingly ties together two threads, Zen practice and haiku.” —Spirituality & Health Known to many as the study of quiet stillness and introspection, Zen Buddhism distinguishes itself through brilliant flashes of insight and its terseness of expression. In River of Heaven these concepts and pillars lend themselves to an exploration of Haiku, one of the most delicate and interpretive poetic forms in the world. The haiku verse form, with its rigid structure and organic description is a superb means of studying Zen modes of thought because its seventeen syllables impose a limitation that confines the poet to vital experience. In Haiku as in Buddhism, the silences are as expressive as the words. In this volume, American Senior Zen Roshi Robert Aitken gives new insight into Haiku by poetic masters Basho, Issa, Buson, and Shiki. In presenting themes from Haiku and from Zen literature, Aitken illuminates the relationship between the two. Readers are certain to find this an invaluable and enjoyable experience for the remarkable revelation it offers. “I am grateful for Robert Aitken’s enthusiastic sharing of poems in The River of Heaven, together with his rich personal and cultural perspectives. It is a book where the author joyfully calls each of us as readers to share in the transcendent joys of haiku.” —Juxtapositions “Aitken mines the meanings in these brief gems about nature, impermanence, travel, awareness, silence, beauty, being present, the turn of the seasons, and much more.” —Spirituality & Practice

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.

A uniquely playful and incisive collection of Zen teaching stories from a beloved American master.A modern classic, now in a new expanded edition. In the tradition of the great koan collections and the records of ancient masters, Robert Aitken distills a lifetime of teaching down to its essence. Intriguing and deceptively simple, Zen Master Raven is a brilliant encapsulation of Zen in over a hundred koan-like encounters alongside many charming illustrations. Featuring curious beginners like Mallard and Mole and profound teachers like Brown Bear, Moose Roshi, and Zen Master Raven himself, this classic of contemporary Zen and will inspire seekers for generations to come.

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.