S. Kim R. Williams - Böcker

Comprehensive, up-to-date, and user-centered one-stop reference on the principles and applications of Field-Flow Fractionation (FFF), a highly versatile separation technology Field-Flow Fractionation: Principles and Applications offers a comprehensive and topical one-stop reference on Field-Flow Fractionation (FFF), an important separation technique which has been proven successful in the analysis of natural and engineered nanoparticles, pharmaceuticals, proteins, polymers, soils, and food. After introductory chapters on theory, principles, and instrumentation, a tutorial-style user guide addresses typical users’ questions and problems. In the application part, the separation of all relevant particle classes is discussed by international experts. Sample topics covered in Field-Flow Fractionation: Principles and Applications include: Historical perspectives of the technique, normal versus steric versus hyperlayer modes, retention resolution, and fractionation powerFFF techniques, including flow (asymmetric, frit inlet, and outlet), sedimentation, thermal, electric, and other novel techniques such as 2DHow to determine if FFF is the correct choice and if it is being implemented correctly, along with general optimization strategies including carrier fluid, calibration, and reproducibilitySample overloading and recovery, detector selection, troubleshooting, and performance of other fractionation techniquesBoth beginners and experienced chemists and researchers can confidently rely on Field-Flow Fractionation: Principles and Applications to confirm their own understanding and to improve their FFF methods and interpretation of their results.

This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.