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Beskrivning
This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems.
From the reviews: "I recommend this book to every FFF user and analytical chemist, and to any researcher, post-graduate students, and academics working with biopolymers. ... This book ... is essential reading for every current and potential FFF user. It discusses, in depth, current and potential applications, the superiority of FFF over other methods ... . This book is certainly a precious help for all scientists in the various fields of (biomedical) research, cell biology, food science, membrane technology, and environmental science, to name a few." (Patrice Castignolles, Analytical and Bioanalytical Chemistry, Vol. 404, 2012)
Innehållsförteckning
Introduction.- 1 Flow FFF - Basics and Key Applications.- 2 Assessing protein-ultrafiltration membrane interactions using flow field-flow fractionation.- 3 Hollow-fiber flow field-flow fractionation: a pipeline to scale down separation and enhance detection of proteins and cells.- 4 Two-Dimensional Separation for Proteomic Analysis.- 5 Field-Flow Fractionation in Therapeutic Protein Development.- 6 Assessing and Improving Asymmetric Flow Field Flow Fractionation of Therapeutic Proteins.- 7 Studies of loose protein aggregates by flow Field-Flow Fractionation (FFF) coupled to Multi-Angle Laser Light Scattering (MALLS).- 8 Field Flow Fractionation for Assessing Biomolecular Interactions in Solution.- 9 Flow Field Flow Fractionation: Analysis of Biomolecules and Their Complexes.- 10 Analysis of prions by field-flow fractionation.- 11 Multifunctionalized Particles for Biosensor Use.- 12 Starch and other polysaccharides.- 13 The use of field-flow fractionation for the analysis of drug and gene delivery systems.- 14 Characterization of liposomes by FFF.- 15 Mammalian Cell Sorting with Sedimentation Field Flow Fractionation.- 16 Isolation and characterization of cells by Dielectrophoretic Field-Flow Fractionation.- 17 Field flow fractionation coupled to inductively coupled plasma-mass spectrometry (FFF-ICP-MS): Methodology and application to environmental nanoparticle research.