Sebastian Boegel - Böcker

This volume focuses on a variety of in silico protocols of the latest bioinformatics tools and computational pipelines developed for neo-antigen identification and immune cell analysis from high-throughput sequencing data for cancer immunotherapy. The chapters in this book cover topics that discuss the two emerging concepts in recognition of tumor cells using endogenous T cells: cancer vaccines against neo-antigens presented on HLA class I and II alleles, and checkpoint inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Bioinformatics for Cancer Immunotherapy: Methods and Protocols is a valuable research tool for any scientist and researcher interested in learning more about this exciting and developing field.

This volume focuses on a variety of in silico protocols of the latest bioinformatics tools and computational pipelines developed for neo-antigen identification and immune cell analysis from high-throughput sequencing data for cancer immunotherapy.

The book explores updated as well as novel in silico tools, resources, and wet lab protocols for HLA typing, including determination of the HLA class I and class II type of an individual in clinical work and research, such as in transplantation medicine and vaccine development in the context of infectious diseases or cancer immunotherapies.

This volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods. The chapters in this book discuss high-throughput methods for HLA typing; wet lab protocols; microarray data and its uses; in silico tools for the identification of HLA alleles from DNA and RNA next-generation-sequencing data, as well as HLA haplotype frequency estimation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, HLA Typing: Methods and Protocols is a valuable resource for any researcher interested in learning more about this developing field.