Shelley D. Minteer – författare

Introducing the topic of electrochemistry for non-electrochemists, Electrochemical Systems provides an overview of the fundamentals of electrochemical theory and techniques along with an appreciation for its wide range of applications. Covering both common topics (batteries, corrosion, and fuel cells) along with new topics (membranes and proteins), introductory chapters provide students and practitioners with the skills to be able to "read" cyclic voltammograms, linear sweep voltammetry, and other various fundamental terms. Chapters are arranged in order of ascending complexity of the system.

This third edition provides new and updated methods on enzyme stabilization. Chapters focus on protocols for enzyme stabilization, enzyme immobilization, and  the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Enzyme Stabilization and Immobilization: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers in the field.

This third edition provides new and updated methods on enzyme stabilization. Chapters focus on protocols for enzyme stabilization, enzyme immobilization, and  the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Enzyme Stabilization and Immobilization: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers in the field.

An authoritative resource introducing the fundamentals of enzymatic and microbial electrocatalysis In Bioelectrocatalysis: From Electron Transfer Processes to Emerging Technological Applications, a team of distinguished researchers delivers an up-to-date discussion of fundamental concepts in bioelectrocatalysis and its applications. The authors offer a comprehensive treatment of the foundations of bioelectrocatalysis. Beginning with a comparison of enzymatic and microbial electrocatalysis, the book goes on to explore the differences between direct and mediated bioelectrocatalysis and the challenges presented by promoting extracellular electron transfer. Bioelectrocatalysis presents detailed and accurate information on the approaches and techniques used to study the electron transfer processes and to confirm the type of electron transfer taking place. Readers will also find chapters dedicated to common and emerging applications of bioelectrocatalysis, including glucometers and glucose monitors, biosensors, and biofuel cells. Inside the book: A thorough introduction to electron transfer in enzymatic bioelectrocatalysisComprehensive explorations of glucometers, continuous glucose monitors, ex-situ biosensors, biofuel cells, and biosolar cellsPractical discussions of microbial electrochemical technologies for used water treatmentComplete treatments of electrosynthesis and wearable and implantable devicesPerfect for academic researchers and industrial scientists working in (bio)electrochemistry, catalysis, and chemical synthesis, energy materials, and sensors, Bioelectrocatalysis will also benefit advanced undergraduate and graduate students studying in any of those fields.

An authoritative resource introducing the fundamentals of enzymatic and microbial electrocatalysis In Bioelectrocatalysis: From Electron Transfer Processes to Emerging Technological Applications, a team of distinguished researchers delivers an up-to-date discussion of fundamental concepts in bioelectrocatalysis and its applications. The authors offer a comprehensive treatment of the foundations of bioelectrocatalysis. Beginning with a comparison of enzymatic and microbial electrocatalysis, the book goes on to explore the differences between direct and mediated bioelectrocatalysis and the challenges presented by promoting extracellular electron transfer. Bioelectrocatalysis presents detailed and accurate information on the approaches and techniques used to study the electron transfer processes and to confirm the type of electron transfer taking place. Readers will also find chapters dedicated to common and emerging applications of bioelectrocatalysis, including glucometers and glucose monitors, biosensors, and biofuel cells. Inside the book: A thorough introduction to electron transfer in enzymatic bioelectrocatalysisComprehensive explorations of glucometers, continuous glucose monitors, ex-situ biosensors, biofuel cells, and biosolar cellsPractical discussions of microbial electrochemical technologies for used water treatmentComplete treatments of electrosynthesis and wearable and implantable devices Perfect for academic researchers and industrial scientists working in (bio)electrochemistry, catalysis, and chemical synthesis, energy materials, and sensors, Bioelectrocatalysis will also benefit advanced undergraduate and graduate students studying in any of those fields.

An authoritative resource introducing the fundamentals of enzymatic and microbial electrocatalysis In Bioelectrocatalysis: From Electron Transfer Processes to Emerging Technological Applications, a team of distinguished researchers delivers an up-to-date discussion of fundamental concepts in bioelectrocatalysis and its applications. The authors offer a comprehensive treatment of the foundations of bioelectrocatalysis. Beginning with a comparison of enzymatic and microbial electrocatalysis, the book goes on to explore the differences between direct and mediated bioelectrocatalysis and the challenges presented by promoting extracellular electron transfer. Bioelectrocatalysis presents detailed and accurate information on the approaches and techniques used to study the electron transfer processes and to confirm the type of electron transfer taking place. Readers will also find chapters dedicated to common and emerging applications of bioelectrocatalysis, including glucometers and glucose monitors, biosensors, and biofuel cells. Inside the book: A thorough introduction to electron transfer in enzymatic bioelectrocatalysisComprehensive explorations of glucometers, continuous glucose monitors, ex-situ biosensors, biofuel cells, and biosolar cellsPractical discussions of microbial electrochemical technologies for used water treatmentComplete treatments of electrosynthesis and wearable and implantable devices Perfect for academic researchers and industrial scientists working in (bio)electrochemistry, catalysis, and chemical synthesis, energy materials, and sensors, Bioelectrocatalysis will also benefit advanced undergraduate and graduate students studying in any of those fields.

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications.

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.